Repair mask containinghigh concentration human mesenchymal stem cell secretion active factors and preparation method thereof

A technology of mesenchymal stem cells and active factors, which is applied in the field of repairing masks containing high concentrations of human mesenchymal stem cells secreting active factors and its preparation, can solve the problems of repairing facial skin that have not been reported, so as to enhance skin repair ability and promote blood vessel repair. Effects of regeneration and wound healing

Inactive Publication Date: 2017-09-08
TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there have been reports on the application of active factors secreted by human mesenchymal stem cells to

Method used

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  • Repair mask containinghigh concentration human mesenchymal stem cell secretion active factors and preparation method thereof
  • Repair mask containinghigh concentration human mesenchymal stem cell secretion active factors and preparation method thereof
  • Repair mask containinghigh concentration human mesenchymal stem cell secretion active factors and preparation method thereof

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preparation example Construction

[0022] The preparation method of the repairing mask containing high-concentration human mesenchymal stem cell-secreted active factor of the present invention comprises the following steps:

[0023] (1) Human mesenchymal stem cell culture: P2-P6 human mesenchymal stem cells were selected from the cell bank for resuscitation, the inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was the maximum fusion 50%-60%, 35%-45%, 20%-30% of the temperature; the cells are inoculated and cultured in a carbon dioxide incubator at a temperature of 37°C and a volume ratio of carbon dioxide of 5%;

[0024] (2) When the fusion degree of the first batch of cells reaches 70%-80%, wash the cells twice with normal saline, and perform starvation culture with compound electrolyte injection;

[0025] (3) After 12-24 hours of starvation culture, the first batch of cell confluence reaches 90%-95%, collect the cell culture supernatant, ...

Embodiment 1

[0039] (1) Culture of human umbilical cord mesenchymal stem cells: P4 human umbilical cord mesenchymal stem cells were selected from the cell bank for recovery, the total number of cells was 4.2*10 7 . The inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was 60% of the maximum fusion degree (the number of inoculated cells per bottle: 9*10 6 , inoculate three bottles of cells), 40% (the number of cells inoculated in each bottle: 6*10 6 , two bottles of inoculated cells), 20% (number of inoculated cells: 3*10 6 , inoculate a bottle of cells), the culture system is 40ml. The complete medium used for culturing the cells is phenol red-free DMEM / DF12 and 10% fetal calf serum by volume, and the bottle for inoculating the cells is a T-175 culture flask. After the cell inoculation is completed, place it in a carbon dioxide incubator for cultivation at a temperature of 37°C and a carbon dioxide concentration of 5...

Embodiment 2

[0050] (1) Culture of human adipose-derived mesenchymal stem cells: P5 human adipose-derived mesenchymal stem cells were recovered from the cell bank, and the total number of cells was 3.84*10 7 . The inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was 50% of the maximum fusion degree (the number of inoculated cells per bottle: 8*10 6 , inoculate three bottles of cells), 35% (number of cells inoculated in each bottle: 5.6*10 6 , two bottles of inoculated cells), 20% (number of inoculated cells: 3.2*10 6 , inoculate a bottle of cells), the culture system is 40ml. The complete medium used for culturing the cells is AMEM without phenol red and 10% fetal bovine serum by volume, and the bottle for inoculating cells is T-175 culture flask. After the cell inoculation is completed, place it in a carbon dioxide incubator for cultivation at a temperature of 37°C and a carbon dioxide concentration of 5%;

[0051...

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Abstract

The invention discloses a repairing facial mask containing high-concentration human mesenchymal stem cells secreting active factors and a preparation method thereof. Human mesenchymal stem cells secrete various and large amounts of cytokines during the culture process, and these cytokines can penetrate deep into the basal layer of the skin to promote Skin tissue differentiation, angiogenesis, and growth of granulation tissue promote structural reconstruction and regeneration of damaged skin tissue, promote the removal of aging cells and regeneration of cells, prevent the loss of skin moisture, and thus maintain and restore the youthful state of the skin. Compared with the traditional mask, the repair mask based on the active factors secreted by mesenchymal stem cells can fundamentally solve the aging problem of the skin, and does not contain any chemical repair ingredients, and has no toxic side effects. In the present invention, the human mesenchymal stem cell secretion is extracted after repeated starvation culture, and contains compound electrolyte injection and other components. It can be used to relieve and repair skin dryness and aging problems caused by various reasons.

Description

technical field [0001] The invention relates to a repairing mask, in particular to a repairing mask containing high-concentration human mesenchymal stem cell-secreted active factors and a preparation method thereof. Background technique [0002] Mesenchymal stem cell (MSC) is a kind of stem cell, named for its ability to differentiate into mesenchymal tissue. MSCs originate from mesoderm mesenchyme, and mainly exist in connective tissue and organ mesenchyme throughout the body. Mesenchymal stem cells are widely distributed in fetal and adult bone marrow, fat, umbilical cord, and umbilical cord blood, among which the umbilical cord-derived mesenchymal stem cells are of high quality, pure and large in number. Mesenchymal stem cells have the common characteristics of stem cells, that is, the ability of self-renewal, multi-directional differentiation, tissue repair, immune regulation and homing. Mesenchymal stem cells have subpluripotent differentiation potential, and can be i...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/02A61Q19/08A61Q19/00A61Q19/02A61K8/34A61K8/37A61K8/41A61K8/64A61K8/73A61K8/81A61P17/02
CPCA61K8/99A61K8/0212A61K8/345A61K8/37A61K8/41A61K8/64A61K8/735A61K8/8147A61K8/8152A61K2800/5922A61Q19/004A61Q19/02A61Q19/08
Inventor 高婧彧张冰晶鲁振宇韩洪起秦臻熊亮徐悦黄文敬
Owner TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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