Method for detecting lung infection pathogenic bacteria by adopting visual gene chip

Abstract

The invention discloses a method for detecting pathogenic bacteria of pulmonary infection by a visualized gene chip, comprising the following steps: S1 determining the spectrum of pathogenic bacteria of pulmonary infection based on clinical practice; S2 making the detection target according to the specific gene of the bacteria; S3 designing general primers and probes S4 chip preparation; S5 target gene amplification; S6 chip hybridization and washing; S7 gene chip visual detection; S8 optimization conditions, establishment of methods; S9 clinical verification; S10 comparison with bacterial culture; Chip detection. Beneficial effects of the present invention: realize rapid, sensitive, and high-throughput detection of important pathogenic microorganisms of common clinical respiratory infection flora, provide evidence for clinical disease diagnosis and anti-infection treatment of acute and severe respiratory infectious diseases; provide emergency, prevention and control Provide effective and efficient screening means and methods.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for detecting pathogenic bacteria of lung infection with a visualized gene chip. Background technique [0002] For a long time, acute severe pulmonary infection caused by respiratory pathogens has been an important disease that threatens human health. If it cannot be treated in time and effectively, it will not only cause respiratory failure, but also lead to multiple organ dysfunction, which seriously threatens the lives of patients. , The bacterial spectrum of lung infection has changed, which is a severe challenge for current medical workers. Rapid and accurate detection of pathogenic bacteria is the premise and key to clinical diagnosis and treatment of pulmonary infection. It is of great significance to reduce patient mortality, hospitalization time, hospitalization costs and waste of medical resources. [0003] At present, the detection and identification meth...

AI Technical Summary

Problems solved by technology

At present, the fluorescence detection method is a routine detection method for gene chips. The method is mature and simple, and can meet the general detection requirements. However, organic fluorescent dyes have their own shortcomings that are difficult to overcome: easy quenching, low signal intensity, etc., and are stable in various environments. Poor performance, which affects the uniformity of the detection signal and the reproducibility of the experiment. Its biggest disadvantage is that the detection equipment is expensive, bulky, and the detection cost is high, which limits the application and promotion of gene chips in clinical and on-site detection. Bottlenecks facing technology development

But so far, there has not been any report on the visualization of gene chips for lung infection pathogen screening based on clinical pathogenic bacteria spectrum.

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