Non-invasive high-throughput methylation in colorectal cancer diagnosis, research and treatment method

Abstract

A non-invasive high-throughput methylation in colorectal cancer diagnosis, research and treatment method is disclosed, and is characterized in that on the basis of high-throughput parallel methylation detection combinatorial sequencing and cfRNA information, DNA methylation of a subject is detected to produce a methylation spectrum of the subject and establish specific CpG island sites contained in SEPT9, MLH1, DKK2 and APC genes, methylation levels of CGI and CpG islands of which identification genes are in promoter regions are used as colorectal cancer diagnosis markers and clinical targets; a specific anti methylcytosine antibody is used for recognization and binding to methylated DNA fragments, a methylation second-generation sequencing library can be established by immunity precipitation, target sites are enriched, a methylation spectrum is compared with standard methylation spectrums, methylation site sequencing bioinformation is analyzed by the ''waterfall'' mechanism to determine whether a sample has cancer, and by combination of cfRNA and methylation site comprehensive analysis, the cancer can be determined and located.

Description

technical field [0001] The invention relates to biotechnology gene methylation detection, in particular to a non-invasive high-throughput methylation colon cancer diagnosis, research and treatment method. Background technique [0002] In recent years, epigenetic research has become a new direction in cancer research. In 1983, scientists first discovered that there is a close relationship between DNA methylation and cancer. Riggs and Jones found that, relative to normal cells, cancer cell genomes exhibit hypomethylation. The difference in the methylation status of each CpG site on genomic DNA constitutes the genomic DNA methylation profile, and abnormal methylation of genomic DNA can lead to the occurrence and development of various human diseases. A large number of research results have shown that abnormal changes in epigenetic modification are closely related to cancer, and genome-wide epigenetic modification has become a new marker of cancer. The latest point of view is...

AI Technical Summary

Problems solved by technology

In conclusion, although DNA methylation detection is recognized as an early cancer detection method, the sensitivity and accuracy of the current technology cannot reach the level of clinical use.

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