Application of KRAS (kirsten ratsarcoma viral oncogene) serving as biomarker in pancreatic cancer
A technology for pancreatic cancer and uses, applied in the field of KRAS as a biomarker in pancreatic cancer, can solve problems to be studied and so on
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[0044] 1. Sample Collection
[0045]Preoperative venous blood was collected using ethylenediaminetetraacetic acid tubes (Vacutainer blood collection tubes, BD Company, USA), and the samples were sent to Ankeji Biotechnology Co., Ltd. (Shanghai, China) at room temperature for plasma separation. All 85 patients underwent 10ml whole blood sample collection. Blood samples for plasma and white blood cell (WBC) samples were drawn on the day of surgery. The results of cfDNA sequencing are not provided to patients, and treatment decisions are not made based on cfDNA results.
[0046] 2. Extraction of cfDNA and genomic DNA
[0047] Peripheral blood (10 ml per sample) was centrifuged at 4° C., 1600 g, for 10 minutes. Plasma and buffy coat (white blood cell layer) were collected and stored at -70°C. cfDNA was extracted from 3-5 ml plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen). gDNA (genomicDNA) was extracted from white blood cells using the QIAamp DNA Blood Maxi kit ...
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