Alcohol-precipitation preparation method of colquhoumia root extract

Abstract

The invention discloses an alcohol-precipitation preparation method of colquhoumia root extract. The alcohol-precipitation preparation method comprises the following steps: cutting colquhoumia roots into thin slices, and adding water and decocting for 2 to 5 times; keeping decoction obtained by each time of decocting; combining all the obtained decoction and filtering; heating at temperature of 40 DEG C to 60 DEG C and concentrating to obtain clear extract with the relative density of 1.05 to 1.07; cooling the clear extract to be 25 DEG C to 35 DEG C; adding 75 percent to 85 percent ethanol into the obtained clear extract; adding while stirring until the content of the ethanol reaches 50 percent to 80 percent, wherein the adding speed of the ethanol is 13000kg / min.m<2> to 14000kg / min.m<2> and the stirring speed is 25r / min to 35r / min; standing; after freezing and precipitating in a low-temperature environment at 2 DEG C to 10 DEG C, carrying out solid-liquid separation and filtering; recycling the ethanol in obtained filtrate and heating and concentrating the filtrate to obtain thick extract with the relative density of 1.30 to 1.35. The colquhoumia root extract is prepared by utilizing the method disclosed by the invention; the clear extract has no flocculent inclusions when the ethanol is added, and the precipitation effect is very good; the yield of the extract is improved to be 9.0 percent or more on the basis that the highest yield of a traditional process is 6.5 percent; a stable process is formed, and the production cost is reduced by more than 1 / 3.

Description

technical field [0001] The invention relates to a preparation method of alcohol precipitation of traditional Chinese medicine immunosuppressant extract, in particular to an alcohol precipitation preparation method of Huobahuagen extract. Background technique [0002] Huobahuagen Tablet is a traditional Chinese medicine immunosuppressant made from the traditional Chinese medicine Huobahuagen as the main raw material. Because of its good anti-inflammatory and immunosuppressive effects, it has been widely used clinically to treat various autoimmune diseases , such as rheumatoid arthritis, lupus erythematosus, chronic nephritis, psoriasis, etc. The preparation of Huobahua root extract is the necessary procedure for the production of Huobahua root slices. [0003] The preparation method of Huobahuagen extract mainly adopts the traditional method of water decoction and alcohol precipitation, that is, in the process of alcohol precipitation, the clear paste is cooled to about 60°C...

AI Technical Summary

Problems solved by technology

[0006] At the same time, according to the current technology to make Huobahuagen extract, add ethanol at about 65°C in the clear paste. Although ethanol can be dissolved quickly, the amount of ethanol is huge and the production cost is high

If ethanol is added when the temperature of the clear ointment is lower than 60°C, the ethanol cannot be effectively dissolved. Not only does it take a long time to work, but also the obtained alcohol precipitated liquid has more impurities, and the quality of the prepared Huobahua extract is poor, which cannot meet the medicinal requirements. need

[0007] Practice has shown that during the alcohol precipitation process, the greater the concentration of ethanol added, the faster the precipitation of impurities during the alcohol precipitation process, and the less impurities contained in the final extract. In order to meet the needs of medicine, according to the current technology level, the concentration of It is more than 90% ethanol, but adding more than 90% ethanol makes the consumption of ethanol very large, and the difficulty of ethanol recovery is also greatly increased, which is not conducive to cost control

[0008] In the prior art, when ethanol is added or after it is added, the ethanol precipitation liquid will be stirred, but the stirring speed is difficult to control. If the stirring speed is too fast, the precipitated particles generated by impurities will be too small, which is difficult to filter and cannot achieve the removal of impurities. Therefore, the safety of the product cannot be guaranteed; if the stirring speed is too slow, due to the high concentration of ethanol added, the local concentration of ethanol in the liquid medicine will be too high, and a large number of wraps will be formed to wrap the active ingredients, resulting in The loss of the sediment will also cause the sediment to stick together, making it difficult to filter and separate

Since the alcohol precipitation process of Huobahuagen extract has no specific parameters in the above-mentioned links, the operation process is very random, which not only leads to large fluctuations in the yield of the extract, but also extremely unstable product quality

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