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Method for extracting glycotropic protein from seaweed

A seaweed protein and seaweed technology, which is applied in the field of extracting glycoproteins from seaweed, can solve the problem of extracting less glycoproteins, and achieve the effects of easy purification, convenient dehydration, and reduced energy consumption

Active Publication Date: 2014-07-30
SHENZHEN XINGQILING FOOD TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively few research reports on the extraction of glycoproteins from seaweed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting glycoprotein from seaweed, comprising the steps of:

[0025] a. Raw material processing: wash 10kg of seaweed, add 10kg of pure water and mince it;

[0026] b. Cell crushing: Put the minced seaweed into a high-pressure tank, pressurize to 3kgf / cm2, and keep it for 10 minutes to make the pressure uniform, and then suddenly reduce the pressure to 0kgf / cm2 in 0-3 seconds, filter, The filtrate was ultrasonically treated for 30 minutes at a temperature of 40°C and a power of 300W, and filtered to obtain a seaweed protein extract;

[0027] c. Protein crude extraction: add ammonium sulfate solution to the obtained seaweed protein extract, so that the saturation of ammonium sulfate reaches 25%, let it stand for 10 minutes, continue to add ammonium sulfate solution, so that the saturation of ammonium sulfate reaches 50%, Stand still for 15 minutes, collect the precipitate;

[0028] d. Protein purification: pass the collected precipitate through a model ...

Embodiment 2

[0031] A method for extracting glycoprotein from seaweed, comprising the steps of:

[0032] a. Raw material processing: wash 10kg of green algae and add 40kg of pure water to mince;

[0033] b. Cell crushing: Put the minced seaweed into a high-pressure tank, pressurize to 3kgf / cm2, and keep it for 20min to make the pressure uniform, then suddenly reduce the pressure to 0kgf / cm2 in 0-3 seconds, filter, The filtrate was ultrasonically treated for 20 minutes, the action temperature was 45°C, and the power was 200W, and filtered to obtain the seaweed protein extract;

[0034] c. Protein crude extraction: Add ammonium sulfate solution to the obtained seaweed protein extract, so that the saturation of ammonium sulfate reaches 25%, let it stand for 15 minutes, continue to add ammonium sulfate solution, so that the saturation of ammonium sulfate reaches 50%, Stand still for 10 minutes, collect the precipitate;

[0035] d. Protein purification: Pass the collected precipitate through ...

Embodiment 3

[0038] A method for extracting glycoprotein from seaweed, comprising the steps of:

[0039] a. Raw material processing: wash 10kg of red algae, add 20kg of pure water and mince;

[0040] b. Cell crushing: Put the minced seaweed into a high-pressure tank, pressurize to 3kgf / cm2, and keep it for 15 minutes to make the pressure uniform, and then suddenly reduce the pressure to 0kgf / cm2 in 0-3 seconds, filter, The filtrate was ultrasonically treated for 25 minutes at a temperature of 50°C and a power of 250W, and filtered to obtain a seaweed protein extract;

[0041] c. Protein crude extraction: Add ammonium sulfate solution to the obtained seaweed protein extract, so that the saturation of ammonium sulfate reaches 25%, let it stand for 15 minutes, continue to add ammonium sulfate solution, so that the saturation of ammonium sulfate reaches 50%, Stand still for 10 minutes, collect the precipitate;

[0042] d. Protein purification: pass the collected precipitate through a model S...

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PUM

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Abstract

The invention discloses a method for extracting glycotropic protein from seaweed. The method for extracting the glycotropic protein from the seaweed comprises the following steps: preparing raw materials, carrying out ultrasonic wave treatment, carrying out high pressure treatment and protein crude extraction, purifying protein, and drying, so that the glycotropic protein product is obtained. Compared with the prior art, the method for extracting the glycotropic protein from the seaweed has the advantages that product content is high and time is greatly shortened.

Description

technical field [0001] The invention relates to a method for preparing a glycoprotein, in particular to a method for extracting a glycoprotein from seaweed. Background technique [0002] Seaweed grows in the ocean and is a cryptogamous plant. No roots, stems and leaves in the fundamental sense, and reproduce through robes. There are some seaweeds that can be used as medicine, such as kelp, seaweed, wakame, stone cauliflower, asparagus and so on. The most common types of seaweed are large seaweeds, green algae, red algae, and brown algae. A distinct growth zone is formed within a water depth of 50 meters. Seaweed growing on the high tide line is exposed to the air for a long time, while seaweed below the low tide line cannot be exposed to the air for a long time, so it cannot grow near the coastline. For example, fucus, macroalgae, sea sacs and kelp can only grow in cold water below 18 degrees. They are generally considered to be simple plants. Because of the simple stru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/405C07K1/36C07K1/34C07K1/30C07K1/16
CPCC07K14/405
Inventor 归三岷
Owner SHENZHEN XINGQILING FOOD TECH CO LTD
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