Portable pathogenic bacterium nucleic acid extraction device and detection method

Abstract

The invention discloses a portable pathogenic bacterium nucleic acid extraction device and a detection method. The device mainly extracts a nucleic acid according to the working principle of the nucleic acid extraction device, and comprises three functional units of a constant temperature unit, a man-machine interaction unit and a power supply unit, wherein the constant temperature unit provides an appropriate constant temperature reaction environment for a system, so that a pathogenic bacterium is rapidly hydrolyzed and eluted, the designed constant temperature unit provides the settable high-precision temperature environment for the system, the man-machine interaction unit provides operation functions of setting and displaying the temperature value, the reaction time and a reaction channel for a user, the power supply unit meets the requirements on outdoor detection, and the paper designs two power supply modes including a power adapter and a battery.

Description

technical field [0001] The invention relates to the field of automatic control, in particular to a portable pathogenic bacteria nucleic acid extraction device and detection method. Background technique [0002] In recent years, food safety incidents have occurred frequently, such as the listeriosis incident in France, the salmonella contamination incident in the United States, the bird flu incident in Hong Kong, and the recent H1N1 incident. Food safety issues have become a worldwide problem that cannot be ignored. . In order to prevent the original food with pathogenic bacteria from flowing into production from the source, and to check the food that is about to enter the market from the terminal, there is an urgent need for rapid detection technology and rapid detection systems, especially portable rapid detection systems for grassroots detection. In the development of portable rapid detection systems, a relatively fatal problem is that it is difficult to apply the nucleic...

AI Technical Summary

Problems solved by technology

[0003] The desktop nucleic acid extraction device used in the laboratory is a large-scale automatic extraction device. The device has a high degree of automation and high extraction efficiency, but it is large in quality and volume, does not have battery-powered power, and is not suitable for on-site nucleic acid extraction of pathogens.

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