Group B streptococcus enzyme linked immunosorbent assay kit

A streptococcal enzyme and immune detection technology, applied in the field of medical detection, can solve the problems of troublesome detection, easy introduction of pollution, affecting detection accuracy, etc., and achieves the effects of convenient operation, easy assembly, and improved work efficiency

Pending Publication Date: 2017-09-22
HANGZHOU LIFEREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the immunoassay method is a commonly used GBS detection method, but in the existing GBS immunoassay method, because each step of the immunoassay involves different processing, usually the sample needs to be processed in different steps by multiple devices, and the detection It is cumbersome, and sometimes it is necessary to transfer samples to different carriers (test tubes) when performing different steps. This process is easy to introduce pollution and affect the detection accuracy

Method used

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  • Group B streptococcus enzyme linked immunosorbent assay kit
  • Group B streptococcus enzyme linked immunosorbent assay kit
  • Group B streptococcus enzyme linked immunosorbent assay kit

Examples

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but not as a basis for limiting the present invention.

[0023] Example. A group B streptococcus ELISA kit, such as figure 1 As shown: it includes a box body 2 with a flip cover 1 on the top, and the box body 2 is sequentially provided with a sample processing area 4 separated by a plunger 3, two first washing areas 5, a detection antibody binding area 6, and two second washing areas Zone 7, luminescent substrate binding zone 8, two third cleaning zones 9 and termination zone 10; the plunger 3 is provided with a plunger hole; the sample processing zone 4 is provided with a sample processing liquid, and the sample processing liquid There are metal stirrers and submicron superparamagnetic immunomagnetic beads coated with GBS antibody; the detection antibody binding area 6 is provided with a binding solution containing biotin-labeled GBS antibody; the lumi...

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Abstract

The invention discloses a group B streptococcus (GBS) enzyme linked immunosorbent assay kit. The group B streptococcus enzyme linked immunosorbent assay kit comprises a box body (2) with a turning cover (1) at the top, wherein the box body (2) is internally provided with a sample treatment region (4), first washing regions (5), a detection antibody combining region (6), second washing regions (7), a luminescent substrate combining region (8), third washing regions (9) and a termination region (10) in sequence, which are separated through plunger pistons (3); the plunger pistons (3) are provided with plunger piston holes; the sample treatment region (4) is provided with a sample treatment solution; the sample treatment solution is internally provided with a metal stirring bar and sub-micron-grade super-paramagnetic immunomagnetic beads coated with a GBS antibody. By adopting the group B streptococcus enzyme linked immunosorbent assay kit, all steps of GBS immunoassay can be integrally finished, and pollution is not easy to introduce; the operation is more convenient and the detection precision is higher.

Description

technical field [0001] The invention relates to a group B streptococcus ELISA detection kit, which belongs to the technical field of medical detection. Background technique [0002] Group B streptococcus (GBS for short), normally inhabits the vagina and rectum, is an opportunistic pathogenic bacteria, and generally normal healthy people are not pathogenic when infected with GBS. [0003] According to statistics, about 10% to 30% of pregnant women are infected with GBS, and 40% to 70% of them will be passed on to the newborn during delivery. If newborns carry this bacterium, about 1% to 3% will have early invasive infection, and 5% of them will cause death. Therefore, the detection of GBS is particularly important. Among them, the immunoassay method is a commonly used GBS detection method, but in the existing GBS immunoassay method, because each step of the immunoassay involves different processing, usually the sample needs to be processed in different steps by multiple dev...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56944
Inventor 华灿忠
Owner HANGZHOU LIFEREAL BIOTECH CO LTD
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