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Method for rapidly detecting concentration of polydopamine stable dispersion

A polydopamine and detection method technology, applied in the direction of color/spectral characteristic measurement, etc., can solve problems such as absorption spectrum extension

Inactive Publication Date: 2017-09-26
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the oxidation of dopamine makes polydopamine have significant absorption capacity for visible light, but the self-polymerization reaction will promote the absorption spectrum of polydopamine to extend to the near infrared region (Acc.Chem.Res.2000,33,307-313)

Method used

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  • Method for rapidly detecting concentration of polydopamine stable dispersion
  • Method for rapidly detecting concentration of polydopamine stable dispersion
  • Method for rapidly detecting concentration of polydopamine stable dispersion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Disperse the prepared polydopamine aqueous dispersion sample 1 (2mg / mL, average particle size 200nm) ultrasonically (90W power) for 30 minutes, and then dilute different multiples to obtain a polydopamine aqueous dispersion of known concentration, with at least 5 Aqueous dispersions of polydopamine with known concentrations of different concentrations ((20μg / mL, 40μg / mL, 60μg / mL, 80μg / mL, 100μg / mL). The absorbance value of the dispersion at 800nm ​​was then detected by an ultraviolet spectrophotometer , To establish a standard curve of the concentration and absorbance of polydopamine aqueous dispersion ( figure 2 ), the concentration of the aqueous dispersion of polydopamine with unknown concentration can be obtained from the standard curve. The concentration of the polydopamine aqueous dispersion sample to be tested needs to be within the range of the standard curve. If it exceeds the test range, the sample concentration needs to be diluted or thickened to the range of...

Embodiment 2

[0030] Disperse the prepared polydopamine ethanol dispersion sample 2 (3mg / mL, average particle size 290nm) ultrasonic (power 80W) for 20 minutes, and then dilute different multiples to obtain a polydopamine ethanol dispersion of known concentration, with at least 5 Polydopamine ethanol dispersions with known concentrations of different concentrations (10μg / mL, 30μg / mL, 50μg / mL, 70μg / mL, 90μg / mL). Subsequently, an ultraviolet spectrophotometer was used to detect the absorbance value at 818 nm, thereby obtaining the concentration of the polydopamine ethanol dispersion liquid according to the standard curve. The concentration of the polydopamine aqueous dispersion sample to be tested needs to be within the range of the standard curve. If it exceeds the test range, the sample concentration needs to be diluted or thickened to the range of the standard curve before testing.

[0031] The concentration of the polydopamine ethanol dispersion obtained for this example is shown in Table 1....

Embodiment 3

[0037] Disperse the prepared polydopamine dimethylformamide dispersion sample 3 (5mg / mL, average particle size of 360nm) ultrasonic (power 80W) for 30 minutes, and then dilute different multiples to obtain a known concentration of polydopamine dimethyl Formamide dispersion, at least 5 different concentrations (20μg / mL, 40μg / mL, 60μg / mL, 80μg / mL, 100μg / mL) of known concentration of polydopamine dimethylformamide dispersion. Subsequently, an ultraviolet spectrophotometer was used to detect the absorbance value at 780 nm, and thus the concentration of the polydopamine dimethylformamide dispersion liquid could be obtained according to the standard curve. The concentration of the polydopamine dimethylformamide dispersion sample to be tested needs to be within the range of the standard curve. If it exceeds the test range, the sample concentration needs to be diluted or thickened to the range of the standard curve before testing.

[0038] The concentration of the polydopamine dimethylfo...

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Abstract

The invention discloses a method for rapidly detecting a concentration of polydopamine stable dispersion. The method comprises the following steps: 1) drawing a standard working curve, namely measuring the absorbancy of polydopamine standard dispersion of multiple different concentrations from a lower concentration to a higher concentration prepared from the prepared polydopamine in a near-infrared light region of 750nm-900nm, and establishing the standard working curve of the polydopamine standard dispersion and the absorbancy; 2) measuring the absorbancy of a to-be-detected polydopamine sample solution, and realizing rapid detection of the polydopamine dispersion of an unknown concentration according to the standard working curve. According to the detection method disclosed by the invention, a method for rapidly detecting the concentration of the polydopamine stable dispersion is established by utilizing the absorption characteristics of the polydopamine in the near-infrared light region, and certain convenience is provided for wide utilization of the polydopamine.

Description

Technical field [0001] The invention belongs to the technical field of material detection, and specifically relates to a method for rapidly detecting the concentration of a stable dispersion of polydopamine. Background technique [0002] Polydopamine is the main component of protein secreted by marine mussel organisms. There are a large number of functional groups such as phenolic hydroxyl, amino and imino groups in its structure. They can be used as the binding site of the reaction and take the form of covalent bonds. The corresponding target molecule can also be fixed in a way, and functional composite materials can also be prepared by chelating with metal ions or transition metal ions through its redox properties. In addition, polydopamine has strong adhesion, can adhere to the surface of various organic and inorganic substrates, and has good stability. At the same time, polydopamine also has excellent characteristics such as optics, electricity, and magnetism, among which th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 李滨刘莹莹
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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