Unlock instant, AI-driven research and patent intelligence for your innovation.

A small molecule chip, its construction method, its application and its detection method

A construction method, small molecule technology, applied in measuring devices, analytical materials, transmittance measurement, etc., can solve the problems of high transportation and storage costs, high production costs, complex antibody preparation process, etc.

Active Publication Date: 2019-11-26
苏州普芯生命科学技术有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has high sensitivity and strength, but it also has certain disadvantages
For example, the antibody preparation process used in the Elisa kit is complicated and the production cost is high; and the antibody is variable and inactivated, the performance is unstable, and the transportation and storage costs are also high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A small molecule chip, its construction method, its application and its detection method
  • A small molecule chip, its construction method, its application and its detection method
  • A small molecule chip, its construction method, its application and its detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1 Construction of Small Molecule Chip

[0075] Take the small molecular compound 2-(1-(2,3-naphthyridine)) ethyl hydrazinecarboxylate hydrochloride, 6-methyl-2-(2-aminoethoxy)methyl-4- (2-Chlorophenyl)-1,4-dihydro-3,5-pyridinedicarboxylic acid methyl ethyl ester, (2S,5R,6R)-3,3-dimethyl-6-(2-phenylacetamido )-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid sodium salt and 4-amino-2-hydroxybenzoic acid, positive control rapamycin, small molecule The compound and the positive control were respectively dissolved in DMSO at a concentration of 10 mmol / L. Pure DMSO was used as a blank control. Using a high-precision automatic spotting instrument, 2 μL of the small molecule compound, the positive control, and the blank control were respectively spotted on the two-dimensional photocrosslinking Place the surface of the chip at 25°C (dry, room temperature) for 1 hour; 2) for 2 exposures, each time for 3 minutes, photo-crosslinking to fix the analyte; soak the ...

Embodiment 2

[0076] Example 2 Construction of Small Molecule Chip

[0077] Take the small molecule compound N-(3-chloro-4-(3-fluorobenzyloxy))phenyl-6-(5-((2-methylsulfonylethylamino)methyl)furan-2-yl ) quinazolin-4-amine, tricyclodecanylamine and (4-(2-chloro-4-nitrophenyl)piperidin-1-yl)(5-methyl-3-phenylisoxazole- 4-yl) ketone, positive control rapamycin, the small molecular compound and the positive control were dissolved in DMSO respectively, the concentration was 5mmol / L, pure DMSO was used as a blank control, and the small molecular compound was respectively taken by using a high-precision automatic spotting instrument. Spot 2 μL of the positive control and blank control on the surface of the three-dimensional photocrosslinking chip, and place it at 30°C (drying room temperature) for 1 hour; 2 ) exposure for 4 times, each time is 3min, photo-crosslinking fixed the object to be tested; soak the chip in analytically pure DMSO, DMF, THF and deionized water respectively, ultrasonic cle...

Embodiment 3

[0078] Example 3 Construction of Small Molecule Chip

[0079] Take the small molecular compound 2-(1-(2,3-naphthyridine)) ethyl hydrazinecarboxylate hydrochloride, 6-methyl-2-(2-aminoethoxy)methyl-4- (2-Chlorophenyl)-1,4-dihydro-3,5-pyridinedicarboxylic acid methyl ethyl ester, (2S,5R,6R)-3,3-dimethyl-6-(2-phenylacetamido )-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid sodium salt, 4-amino-2-hydroxybenzoic acid, N-(3-chloro-4-( 3-fluorobenzyloxy))phenyl-6-(5-((2-methylsulfonylethylamino)methyl)furan-2-yl)quinazolin-4-amine, tricyclodecanylamine and (4-(2-chloro-4-nitrophenyl) piperidin-1-yl) (5-methyl-3-phenylisoxazol-4-yl) ketone, positive control rapamycin, will The small molecule compound and the positive control were dissolved in DMSO at a concentration of 10 mmol / L, and pure DMSO was used as a blank control. Using a high-precision automatic spotting instrument, 1 μL of the small molecule compound, the positive control, and the blank control were respectively...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of a biological chip, and discloses a micro-molecular chip, its structuring method, an application and a detecting method. The micro-molecular chip is formed by chemically bonding a light crosslinking chip and a micro-molecular compound, wherein the micro-molecular compound can be combined with BChE protein or BACE1 protein. The invention further provides a structuring method of the micro-molecular chip. By applying the method, the micro-molecular compound is high in fixing efficiency and good in fixing effect. The invention further provides a method for detecting the BChE protein or BACE1 protein by using the micro-molecular chip; the method is simple in operation and exact in detection result. The micro-molecular chip provided by the invention is used for detecting BChE protein or BACE1 protein in a to-be-detected sample, and has advantages of low cost and stable performance.

Description

technical field [0001] The invention relates to the technical field of biochips, in particular to a small molecule chip, its construction method, its application and its detection method. Background technique [0002] Small molecule chips, also known as small molecule microarrays, are one of the important branches of biochip research. Since the first report of small molecule microarrays in 1999 by Schreiber's research group at Harvard University, small molecule chips have been widely used. At present, the research on small molecule chips mainly has two directions. One is the basic research based on the chemical modification of the surface of small molecule chip carriers and the immobilization strategy of small molecule compounds, and the other is to expand the application range of small molecule chips. In recent years, small molecule chips have been successfully used in the screening of various protein ligands, the interaction between proteins and small molecules, the scree...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/59
CPCG01N21/59G01N2021/5903
Inventor 赵智张振伟何建安彭开美祁婷婷朱劲松
Owner 苏州普芯生命科学技术有限公司