Rapid diagnosis reagent for mild and moderate brain injuries, kit containing same and preparation and detection methods of kit

A technology for rapid diagnosis and brain injury, applied in the field of immunoassays, which can solve problems such as failures and detection methods that do not yet exist

Inactive Publication Date: 2017-09-26
湖州华远生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, based on the practical experience of the inventors of the present application, due to the existence of individual differences, the clinical trials of the above-mentioned single factor or two factors all fa

Method used

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  • Rapid diagnosis reagent for mild and moderate brain injuries, kit containing same and preparation and detection methods of kit
  • Rapid diagnosis reagent for mild and moderate brain injuries, kit containing same and preparation and detection methods of kit
  • Rapid diagnosis reagent for mild and moderate brain injuries, kit containing same and preparation and detection methods of kit

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Embodiment 1

[0017] Rapid diagnostic reagents and kits for patients with moderate to mild brain injury, including capture antibodies, capture antibodies, blocking buffers, standards, labeled antibodies, washing solutions, and chromogenic solutions, etc. The labeled antibody is HRP-labeled secondary antibody. The capture antibodies include monoclonal antibodies prepared separately from S100, GFAP and UCHL1; through the joint rapid detection of S100, GFAP and UCHL1, the detection accuracy and sensitivity can be effectively improved, and the detection of a single factor can be effectively overcome. insufficient. figure 1 It is a schematic diagram of the rapid diagnostic kit for patients with moderate to mild brain injury of the present invention.

[0018] The capture antibody is the corresponding monoclonal antibody obtained by cloning S100, GFAP, and UCHL1 into eukaryotic expression vectors, realizing protein expression in mammalian cells, obtaining corresponding antigens after purification...

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Abstract

The invention belongs to the technical field of immunoassays, and in particular relates to a rapid diagnostic reagent for mild to moderate brain damage, a kit containing the reagent, and methods for preparing and detecting the kit. The reagent and the kit include monoclonal capture antibodies prepared by S100, GFAP, and UCHL1 respectively; the kit also includes detection antibodies, polyclonal antibodies prepared by S100, GFAP, and UCHL1 respectively. The beneficial effects of the present invention are as follows: it can comprehensively detect mild and moderate brain damage, can better judge the physiological and structural changes after brain damage, better carry out differential diagnosis on patients with moderate and mild brain damage, and has high sensitivity and accuracy Strong features, its sensitivity and accuracy are above 96%.

Description

technical field [0001] The invention belongs to the technical field of immunoassays, and in particular relates to a rapid diagnostic reagent for mild to moderate brain damage, a kit containing the reagent, and a preparation and detection method for the kit. Background technique [0002] Traumatic brain injury (TBI) is currently a major public health problem causing death and disability worldwide. There are 235×103 cases of non-fatal craniocerebral trauma hospitalized in the United States every year, and 43.30% of them are left with different degrees of disability. The annual incidence of traumatic brain injury in my country is 783 cases per 100,000 people, of which severe traumatic brain trauma with Glasgow Coma Scale (GCS) score ≤ 8 and post-traumatic amnesia (PTA) > 24 h accounts for about 10%. [0003] More than 1.5 million children and adults suffer concussions in the United States each year, and thousands of military personnel worldwide suffer from mild traumatic br...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/531
CPCG01N33/577G01N33/531G01N2800/28
Inventor 曾小华余跃飞黄行许唐珂张旭亮
Owner 湖州华远生物技术有限公司
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