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Cationic polypeptide nano-aggregate and antibacterial application thereof

A technology of cationic polypeptides and aggregates, which is applied in the application field of preparation and antibacterial direction, and can solve problems such as not easy to degrade

Inactive Publication Date: 2017-09-29
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional antimicrobial peptides usually interact with bacteria as monomers in solution, and monomers are easily degraded in physiological environments and lose certain antibacterial effects, while antimicrobial peptides with aggregate structures are more stable in solution, compared with monomers, It is also not easy to be degraded, so the development of new antimicrobial peptides to self-assemble into aggregate structures has become a current research hotspot

Method used

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  • Cationic polypeptide nano-aggregate and antibacterial application thereof
  • Cationic polypeptide nano-aggregate and antibacterial application thereof
  • Cationic polypeptide nano-aggregate and antibacterial application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Cationic Peptide K 3 (FA) 4 K 3 and K 6 (FA) 4 Aggregate preparation:

[0031] to a concentration of 166 μg mL –1 The cationic polypeptide was added to water at 37°C (pH=7.04), and placed on a shaker to incubate for 40min (360rpm). The cationic polypeptide self-assembled to form nano-aggregates, and the obtained morphology was shown in image 3 . It can be seen that the polypeptide K after incubation 3 (FA) 4 K 3 Self-assembled to form sheet-like nanoaggregates, and the incubated polypeptide K 6 (FA) 4The self-assembly forms relatively uniform spherical nanoaggregates.

Embodiment 2

[0033] Cationic Peptide K 3 (FA) 4 K 3 and K 6 (FA) 4 Antimicrobial applications of nanoaggregates:

[0034] 50 μL E. coli suspension (~5×10 6 cfu mL –1 ) were added to 2mL cationic polypeptide self-assembled nano-aggregate solution, placed in a 37°C constant temperature incubator, and diluted 10,000 times with phosphate buffer every 1h, and 100μL of the diluted suspension was placed in the Lysogeny Broth solid culture medium at 37°C for 18 hours, and count the number of colonies.

Embodiment 3

[0036] The same as in Example 2, only the bacteria in the cationic polypeptide antibacterial step are changed to be Escherichia coli, Salmonella, and Bacillus subtilis, and the resulting bacterial survival rate changes over time. See Figure 4 . It can be seen that under the same conditions, polypeptide K 3 (FA) 4 K 3 and K 6 (FA) 4 The nanoaggregates can kill bacteria, but the peptide K 6 (FA) 4 The nanoaggregates have no bactericidal effect on the Gram-positive bacteria Bacillus subtilis, which can be attributed to the fact that different bacteria have different biological structures and properties.

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Abstract

The invention provides a cationic polypeptide nano-aggregate and antibacterial application thereof, belonging to the field of preparation and application of antibacterial peptide. According to the invention, cationic polypeptide is dispersed in a solution; the cationic polypeptide nano-aggregate is formed through self-assembling by changing the concentration of the cationic peptide, the pH value of the solution, incubation temperature T and incubation time T; and then, the nano-aggregate is applied to sterilization. A solution with a cationic polypeptide concentration of 100 to 480 [mu]g / mL is subjected to inoculation on an oscillator at 35 to 38 DEG C (wherein a pH value is 6.5 to 8.5) for 30 to 60 min (360 rpm) for formation of the nano-aggregate through self-assembling. The nano-aggregate is mixed with a bacterial solution; and the nano-aggregate can kill a variety of bacteria efficiently and quickly, and bactericidal efficiency is up to 100% within 4 h.

Description

technical field [0001] The invention belongs to the field of preparation and application of antibacterial peptides, and in particular relates to the preparation and antibacterial application of cationic polypeptide nano-aggregates. Background technique [0002] The application of penicillin in bacterial infection has freed people from the threat of death from bacterial infection. Subsequently, more and more antibiotics were discovered and used in medical treatment. However, although the rapid development of antibiotics has made outstanding contributions to human health, it is a double-edged sword. Nowadays, with the excessive use of antibiotics, the problem of bacterial resistance is becoming more and more serious. Especially the use of antibiotics in the medical field has made the effect of antibiotics weaker and weaker. In recent years, the emergence of "super drug-resistant bacteria" has seriously threatened human health. Therefore, it is imminent to study new antibacte...

Claims

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Application Information

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IPC IPC(8): C07K14/47A61P31/04
CPCC07K14/4723Y02A50/30
Inventor 刘磊邵辉林琳董明东
Owner JIANGSU UNIV
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