Fiber-array-based multi-channel fluorescence detection system

Abstract

The invention provides a fiber-array-based multi-channel fluorescence detection system. A solid-state optical fiber array receives a fluorescence signal and transmits the signal to a flexible fiber; and the flexible fiber is connected to a PMT detection array and transmits the signal to the PMT array. An upper computer controls the multi-channel PMT array to receive an optical signal and transform the optical signal into an analog signal; and a data acquisition card converts the analog signal into a digital signal and returns the digital signal to a main controller. The fixed fiber is used for replacing the pinhole in the traditional confocal system and the fiber array forms the pinhole surface of the confocal system, thereby realizing super-resolution imaging of the confocal system.

Description

technical field [0001] The invention relates to the technical field of fluorescence signal detection systems, in particular, to a fluorescence detection system based on an array detector. Background technique [0002] Confocal microscopes often place a pinhole and point detector on the imaging detection surface, which effectively suppresses stray light on the non-focus plane, and the pinhole is located at the imaging focal plane. In order to improve the tomographic imaging capability of the confocal system, The pinholes (that is, pinholes) are often set very small. In theory, the smaller the pinhole size, the higher the imaging resolution. However, if the pinhole size is set too small, the light signal passing through the pinhole will be weakened, and the imaging How to reduce the size of the pinhole while ensuring the brightness of the image is one of the concerns of the confocal system. [0003] Using a CMOS camera with tiny pixels to replace the pinhole and point detecto...

AI Technical Summary

Problems solved by technology

But for CMOS cameras, it is point-to-surface imaging, that is, the scanning system scans a sample point, and the image plane of this point will be formed in the imaging system, resulting in a relatively slow imaging speed of the CMOS camera.

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