Preparation method of theanine modified fish scale protein mask

The technology of fish scale protein and fish scale collagen is applied in skin care preparations, pharmaceutical formulations, cosmetic preparations and other directions, which can solve the problems of physiological toxicity, reduce cosmetic grades, human injury, etc. Safety, applicability, and good compatibility

Inactive Publication Date: 2017-10-10
QINGDAO AMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the addition of these additives can improve the antibacterial, anti-mildew, and anti-ultraviolet properties of collagen peptides, they are often physiologically toxic. On the one hand, they will inevitably cause harm to the human body; on the other hand, they will reduce the grade of cosmetics.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The invention provides a preparation method of theanine-modified fish scale protein mask, comprising the following steps:

[0038] (1) fish scale collagen peptide is dissolved in hydrochloric acid solution to obtain protein peptide solution;

[0039] The preparation method of the fish scale collagen peptide is as follows: the fish scales are sequentially treated with sodium carbonate solution, hydrochloric acid solution, sodium hydroxide solution and enzyme preparation, and after filtering and drying, the fish scale collagen peptide is obtained.

[0040]Wherein, the specific steps of the enzyme preparation treatment are: add 16 times the amount of water after acid treatment and alkali treatment, adjust and control the pH value at 7, temperature at 50°C, add neutral protease, the ratio of neutral protease to filter residue 2:100, stir for 1 hour, keep warm for 2 hours, then inactivate neutral protease; control the pH value at 2.0, add papain at 60°C, the ratio of papain ...

Embodiment 2

[0046] The invention provides a preparation method of theanine-modified fish scale protein mask, comprising the following steps:

[0047] (1) fish scale collagen peptide is dissolved in citric acid solution to obtain protein peptide solution;

[0048] The preparation method of the fish scale collagen peptide is as follows: the fish scales are sequentially treated with potassium carbonate solution, citric acid solution, potassium hydroxide solution and enzyme preparation, and after filtering and drying, the fish scale collagen peptide is obtained.

[0049] Wherein, the specific steps of the enzyme preparation treatment are: adding 18 times the amount of water after acid treatment and alkali treatment, adjusting and controlling the pH value at 7.1, temperature at 52 ° C, adding papain, the ratio of papain to filter residue is

[0050] 1:100, stir for 1 hour, keep warm for 4 hours, then inactivate papain; control the pH value at 2.0, add trypsin at 60°C, the ratio of trypsin to f...

Embodiment 3

[0056] The invention provides a preparation method of theanine-modified fish scale protein mask, comprising the following steps:

[0057] (1) fish scale collagen peptide is dissolved in citric acid solution to obtain protein peptide solution;

[0058] The preparation method of the fish scale collagen peptide is as follows: the fish scales are sequentially treated with a solution containing sodium carbonate, a citric acid solution, a sodium hydroxide solution and an enzyme preparation, and after filtering and drying, the fish scale collagen peptide is obtained.

[0059] Wherein, the specific steps of the enzyme preparation treatment are: add 15 times the amount of water after acid treatment and alkali treatment, adjust and control the pH value at 6.5, the temperature at 45 ° C, add papain, the ratio of papain to filter residue is 1 : 100, stir for 1.0h, keep warm for 2h, then inactivate papain; control the pH value at 2.0, add neutral protease at 60°C, the ratio of the neutral ...

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Abstract

The invention relates to the technical field of biology, in particular to a preparation method of a theanine modified fish scale protein mask. The preparation method includes the steps: (1) dissolving fish scale collagen peptide in acid solution; (2) dispersing theanine, photo-initiators and inhibitors in solvents, adding the collagen peptide solution in the step (1), uniformly mixing the solution, adjusting pH (potential of hydrogen) of the solution to range from 5 to 7 and irradiating the solution by ultraviolet rays for 1-5 hours; (3) performing ultrasonic treatment on a product in the step (2) for 0.5-3 hours, filtering the product by a microporous membrane to obtain filtrate and deionizing the filtrate sequentially through anion-cation columns; (4) freeze-drying the product in the step (4) in vacuum to obtain theanine modified fish scale protein peptide; (5) uniformly mixing the theanine modified fish scale protein peptide, film-forming agents, plant extracts, thickening agents and penetrating agents in the solvents to obtain the theanine modified fish scale protein mask. By the aid of the preparation method, the antibacterial and antifungal performance of the fish scale protein peptide is improved, and product safety and applicability are enhanced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of a theanine-modified fish scale protein mask. Background technique [0002] Collagen is the main protein component in skin tissue, accounting for 70% to 80% of the dry weight of the skin. It is produced by fibroblasts in the dermis. Collagen is a white, transparent, unbranched fibril, which itself is A very strong protein that, in young, healthy, sun-damaged skin, forms bundles of slightly curved fibers that are arranged at right angles to each other to form layers above and below, which give the skin firmness Effect. But as the years grow older, the collagen not only decreases in quantity, but also becomes irregular in arrangement, and the dermis becomes thinner; environmental factors make it easier for collagen to dissolve and disappear. cause skin aging. It is precisely because of the loss of collagen that the collagen peptide bond and elastic network sup...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/9794A61K8/9789A61K8/9728A61K8/9706A61K8/02A61Q19/00A61Q19/08A61Q17/00A61Q17/04C12P21/06
Inventor 翟岱青
Owner QINGDAO AMA CO LTD
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