Preparation method of hydroxyapatite drug-carrying microsphere with multi-stage sustained-controlled release effect

A technology of hydroxyapatite and drug-loaded microspheres is applied in the directions of inorganic inactive ingredients, active ingredients of tetracycline, inactive ingredients of polymer compounds, etc., to achieve the effect of mild conditions and simple process

Inactive Publication Date: 2017-10-10
HENAN INST OF ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a hydroxyapatite with multi-level slow and controlled release effect for the problem that simple hydroxyapatite drug-loaded microspheres can only release the drug slowly but not control the release of the drug. The preparation method of the stone-loaded drug-loaded microspheres, and at the same time, the hydroxyapatite drug-loaded microspheres prepared by the present invention have carried out a bionic design on the bone tissue, which can provide better therapeutic effects on infectious bone defects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation method of the hydroxyapatite drug-loaded microspheres with multi-stage slow-controlled release effect of the present embodiment is as follows:

[0024] (1) Prepare 100mL of a gelatin hot solution (90°C) with a concentration of 0.02g / mL, then add 4g of hydroxyapatite powder, mix well, and use a syringe to drop the gelatin / nanoapatite mixed solution drop by drop into liquid nitrogen Gelatin / hydroxyapatite microspheres are obtained in the process;

[0025] (2) The above gelatin / hydroxyapatite microspheres were sintered at 900 °C for 2 h to obtain porous hydroxyapatite microspheres, and then 2 g of porous hydroxyapatite microspheres were immersed in 10 mL of vancous hydrochloric acid with a concentration of 5%. Porous hydroxyapatite drug-loaded microspheres can be obtained by freeze-drying the microspheres after adsorbing the drug solution (-40°C, 24h) in the dispersion liquid of mycin for 24h;

[0026] (3) Prepare 10 mL of a PLA diluted solution with a con...

Embodiment 2

[0028] The preparation method of the hydroxyapatite drug-loaded microspheres with multi-stage slow-controlled release effect of the present embodiment is as follows:

[0029] (1) Prepare 100 mL of hot gelatin solution (90°C) with a concentration of 0.03 g / mL, then add 9 g of hydroxyapatite powder, and after mixing evenly, use a syringe to drop the gelatin / nanoapatite mixed solution dropwise into liquid nitrogen That is, gelatin / hydroxyapatite composite microspheres are obtained;

[0030] (2) The above-mentioned gelatin / hydroxyapatite composite microspheres were sintered at 900 °C for 2 h to obtain porous hydroxyapatite microspheres, and then 4 g of porous hydroxyapatite microspheres were immersed in 10 mL of hydrochloric acid with a concentration of 7.5%. Porous hydroxyapatite drug-loaded microspheres can be obtained by lyophilizing the microspheres after adsorbing the drug solution (-40℃, 24h) in vancomycin dispersion solution for 18h;

[0031] (3) Prepare 10 mL of PLA dilut...

Embodiment 3

[0033] The preparation method of the hydroxyapatite drug-loaded microspheres with multi-stage slow-controlled release effect of the present embodiment is as follows:

[0034] (1) Prepare 100mL of hot gelatin solution (90°C) with a concentration of 0.04g / mL, then add 16g of hydroxyapatite powder, mix well, use a syringe to drop the gelatin / nanoapatite mixed solution dropwise into liquid nitrogen That is, gelatin / hydroxyapatite composite microspheres are obtained;

[0035] (2) The above-mentioned gelatin / hydroxyapatite composite microspheres were sintered at 900 °C for 2 hours to obtain porous hydroxyapatite microspheres, and then 5 g of porous hydroxyapatite microspheres were immersed in 10 mL of hydrochloric acid with a concentration of 10%. Porous hydroxyapatite drug-loaded microspheres can be obtained by lyophilizing the microspheres after adsorbing the drug solution (-40℃, 24h) in vancomycin dispersion solution for 12h;

[0036] (3) Prepare 10 mL of PLA dilute solution wit...

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Abstract

The invention discloses a preparation method of a hydroxyapatite drug-carrying microsphere with a multi-stage sustained-controlled release effect, belonging to the field of biomedical materials. The preparation method comprises the following steps: (1) preparing a hydroxyapatite / chitosan microsphere by virtue of an electrostatic drop generation method, and carrying out high-temperature sintering, so as to obtain a porous hydroxyapatite microsphere; (2) soaking the porous hydroxyapatite microsphere obtained in the step (1) into medical liquid, and carrying out freeze drying, so as to obtain a drug-carrying hydroxyapatite microsphere; and (3) mixing the drug-carrying hydroxyapatite microsphere obtained in the step (2) with PLGA and a drug, and filtering under low-temperature conditions, so as to obtain the drug-carrying hydroxyapatite microsphere with a surface containing PLGA. The preparation method is simple, high in repeatability and applicable to industrial production; and the prepared drug-carrying microsphere is high in drug-carrying quantity, has a long-term sustained-controlled release effect and has relatively wide application prospects in the treatment of chronic infection and inflammation of bone defect.

Description

technical field [0001] The invention belongs to the field of drug sustained-release bone defect repair, and in particular relates to a preparation method and application of hydroxyapatite drug-loaded microspheres with multi-stage sustained and controlled release effects. Background technique [0002] At present, bone defects caused by disease or trauma have brought great inconvenience to people's lives, and bone defects are prone to infection during the treatment process, resulting in infectious bone defects. At present, there are generally two types of treatment methods for infected bone defects: one is to fill the defect area with scaffold materials and intramuscularly inject antibiotics. This method can have a good therapeutic effect on infected bone defects, but there are also antibiotics. The number of treatment times is more, which brings inconvenience to the patient's life, and the utilization rate of antibiotics is low, which is easy to cause nephrosis; another metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K47/02A61K47/42A61K47/34A61K38/14A61K31/65A61P19/08A61P31/00A61L27/22A61L27/12A61L27/56A61L27/54A61L27/50A61L27/34A61L27/58
Inventor 于翔张浩杨柳徐茜卢晓龙赵俊杰
Owner HENAN INST OF ENG
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