A kind of high-efficiency cadmium adsorption fungal agent and its preparation method and application
A fungal agent and adsorbent technology, which is applied in the field of high-efficiency cadmium adsorption fungal agent and its preparation, can solve the problems of high cadmium treatment cost, poor treatment effect, long treatment time, etc. The effect of strong adsorption capacity
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Embodiment 1
[0027] A preparation method for high-efficiency cadmium-adsorbing fungal agent, comprising the following steps:
[0028] (1) Activated bacteria
[0029] Inoculate Hanzhi in PDA solid medium and culture in dark at 28°C. When it grows to 3 / 4 plate, transfer the strain to new PDA solid medium and culture in dark at 28°C;
[0030] (2) When the mycelium of the activated strain grows to a 3 / 4 plate, inoculate the mycelium in a liquid-enriched medium, place it at 28°C, and culture it in dark at 170r / min for 6 days, centrifuge, and remove the filtrate , made; wherein, the rich medium is added 3g of potato dipping powder, 20g of glucose, KH in every liter of nutrient solution 2 PO 4 1g, MgSO 4 0.25g, sucrose 2g and adsorbent 8g.
[0031] Wherein, the adsorbent is prepared by the following method:
[0032] ① Grind coconut shells, rice roots and cottonseed husks to 80 meshes respectively, remove impurities from reeds, rape straws and rice straws, cut to 2 cm, mix, and bake at 90°C ...
Embodiment 2
[0036] A preparation method for high-efficiency cadmium-adsorbing fungal agent, comprising the following steps:
[0037] (1) Activated bacteria
[0038] Inoculate Hanzhi in PDA solid medium and culture in dark at 28°C. When it grows to 3 / 4 plate, transfer the strain to new PDA solid medium and culture in dark at 28°C;
[0039] (2) When the mycelium of the activated strain grows to a 3 / 4 plate, inoculate the mycelium in a liquid-enriched medium, place it at 28°C, and culture it in dark at 170r / min for 6 days, centrifuge, and remove the filtrate , made; wherein, the rich medium is added 3g of potato dipping powder, 20g of glucose, KH in every liter of nutrient solution 2 PO 4 1g, MgSO 4 0.2g, sucrose 1.5g and adsorbent 5g.
[0040] Wherein, the adsorbent is prepared by the following method:
[0041] ① Grind coconut shells, rice roots and cottonseed husks to 80 meshes respectively, remove impurities from reeds, rape straws and rice straws, cut to 2 cm, mix, and bake at 90°C...
Embodiment 3
[0045] A preparation method for high-efficiency cadmium-adsorbing fungal agent, comprising the following steps:
[0046] (1) Activated bacteria
[0047] Inoculate Hanzhi in PDA solid medium and culture in dark at 28°C. When it grows to 3 / 4 plate, transfer the strain to new PDA solid medium and culture in dark at 28°C;
[0048] (2) When the mycelium of the activated strain grows to a 3 / 4 plate, inoculate the mycelium in a liquid-enriched medium, place it at 28°C, and culture it in dark at 170r / min for 6 days, centrifuge, and remove the filtrate , made; Wherein, the enriched medium is adding 5g of potato dipping powder, 23g of glucose, KH in every liter of nutrient solution 2 PO 4 1.5g, MgSO 4 0.3g, 2.5g of sucrose and 8g of adsorbent.
[0049] Wherein, the adsorbent is prepared by the following method:
[0050]① Grind coconut shells, rice roots and cottonseed husks to 80 meshes respectively, remove impurities from reeds, rape straws and rice straws, cut to 2 cm, mix, and ...
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