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Application of osteopontin in preparation or screening of diagnostic reagents for acute-on-chronic liver failure

A technology for acute liver failure and osteopontin, which is applied in the fields of biomedicine and disease diagnosis, and can solve problems such as limitations of timely diagnosis, invasiveness, complex and time-consuming diagnostic methods, etc.

Active Publication Date: 2017-10-17
孔晓妮 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above diagnostic methods are either complex, time-consuming, or invasive, and have great limitations for timely diagnosis.

Method used

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  • Application of osteopontin in preparation or screening of diagnostic reagents for acute-on-chronic liver failure
  • Application of osteopontin in preparation or screening of diagnostic reagents for acute-on-chronic liver failure
  • Application of osteopontin in preparation or screening of diagnostic reagents for acute-on-chronic liver failure

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1. Experimental method

[0058] Peripheral blood samples were collected from 70 patients with confirmed acute-on-chronic liver failure (ACLF+), 67 patients with decompensated liver cirrhosis (ACLF-), 50 patients with hepatocellular carcinoma (HCC), and 30 normal people (Health). A blood sample was collected in a coagulation tube with a volume of about 5ml, centrifuged at 5000rpm for 10min, and the upper clarified serum was absorbed. The concentrations of osteopontin, M30, and M65 in serum were detected by ELISA kits (Osteopontin enzyme-linked immunosorbent assay kit was purchased from R&D Systems, article number: DOST00; M30, M65 enzyme-linked immunosorbent assay kit was purchased from VLVbio company, article number: nr.10011 and nr.10040), MELD, total bilirubin and creatinine concentrations were obtained from patient history data. GraphPad Prism 6.0 software was used to draw the ROC curve, Mann-Whitney test was used for comparison between groups, and Spearman correlat...

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Abstract

The invention belongs to the field of biomedicine and disease diagnosis and particularly relates to application of osteopontin in the preparation or screening of diagnostic reagents for acute-on-chronic liver failure. It is disclosed herein for the first time that osteopontin can act as a biomarker for distinguishing acute-on-chronic liver failure (with diagnostic effect up to 0.9454), significantly higher than M30 (0.7167), M65 (0.8319) and M30 / M65 (0.8100), and almost flush with clinically common index MELD (model for end-stage liver disease) (0.9317).

Description

technical field [0001] The invention belongs to the field of biomedicine and disease diagnosis, and specifically relates to the use of osteopontin for preparing or screening diagnostic reagents for acute-on-chronic liver failure. Background technique [0002] Osteopontin (OPN) is a multifunctional protein widely distributed in various tissues and cells, and plays an important role in immunity, angiogenesis, fibrogenesis and tumor formation. Osteopontin plays an important role in intracellular and intercellular signal transduction by interacting with its ligands integrin, CD44, lipopolysaccharide, vimentin and MyD88, thereby regulating cell proliferation, migration, inflammation, fibrosis and carcinogenesis. At present, a large number of studies have reported that osteopontin plays a key signaling role in the development of acute liver injury, non-alcoholic fatty liver, alcoholic liver, hepatitis, liver fibrosis and cirrhosis, and liver cancer. [0003] Acute-on-chronic live...

Claims

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Application Information

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IPC IPC(8): G01N33/535G01N33/68
CPCG01N33/535G01N33/6893G01N2800/085
Inventor 孔晓妮邬海龙夏强李海闻衍凯
Owner 孔晓妮
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