Method for quickly detecting zearalenone toxin in vegetable oil

A technology of zearalenone and vegetable oil, which is applied in the field of detection, achieves the effects of high promotion and application value, less time-consuming, and simple detection

Inactive Publication Date: 2017-10-20
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no clear standard for the detection of zearalenone toxin in vegetable oil, and zearalenone toxin in edible oil has become an important factor affecting food safety in my count

Method used

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  • Method for quickly detecting zearalenone toxin in vegetable oil
  • Method for quickly detecting zearalenone toxin in vegetable oil
  • Method for quickly detecting zearalenone toxin in vegetable oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The test strips and immunoaffinity column were used to detect zearalenone toxin in vegetable oil samples (commercially available corn oil). Specific steps are as follows:

[0028] 1. Using test strips to detect the content of zearalenone toxin in corn oil samples

[0029] Accurately measure 1g of corn oil sample that has been fully mixed with a pipette, put it in a 50mL centrifuge tube, add 9mL of 70% analytical grade methanol (a solution prepared with analytical grade methanol), vortex at room temperature for 2min, centrifuge at 10000rpm for 5min, and statically Put 100 μL of the supernatant into a 2 mL centrifuge tube, add 1 mL of dilution buffer, mix by inverting up and down for 20 seconds, and then use a test strip to detect the toxin content. The corn oil samples were repeated 3 times and the average value was taken.

[0030] 2. Detection of zearalenone toxin content in corn oil samples by immunoaffinity column

[0031] Use a pipette to accurately measure 4g of ...

Embodiment 2

[0038] The test strips and immunoaffinity column were used to detect zearalenone toxin in vegetable oil samples (commercially available corn oil). The parameters different from Example 1 are:

[0039] Use a pipette to accurately measure 1 g of the fully mixed corn oil sample, place it in a 50 mL centrifuge tube, add 1 mL, 2 mL, and 5 mL of 70% analytical grade methanol respectively, and use a test strip to detect the concentration of the vegetable oil sample (commercially available corn oil). Zearalenone toxin. Each sample was performed three times in parallel.

[0040] The test results showed that the average recoveries of corn oil samples and 70% analytical grade methanol, 1:1, 2:1, 5:1 (mass volume ratio g:mL) were 35.01%, 48.20%, 61.26%, respectively.

[0041] Accurately measure 1g of corn oil sample that has been fully mixed with a pipette, put it in a 50mL centrifuge tube, add 5mL of 70% analytical grade methanol and acetonitrile respectively, and use a test strip to d...

Embodiment 3

[0044] The test strips and immunoaffinity column were used to detect zearalenone toxin in vegetable oil samples (commercially available corn oil). The parameters different from Example 1 are:

[0045] Use a pipette to accurately measure 1g of corn oil sample that has been fully mixed, put it in a 50mL centrifuge tube, add 9mL of 100% and 9mL of 85% analytical grade methanol solution (prepared with analytical grade methanol), and use a test strip to detect the vegetable oil sample (commercially available corn oil) zearalenone toxin. Each sample was performed three times in parallel.

[0046] The test results showed that the average recoveries of corn oil samples and 100% and 85% methanol were 72.14% and 68.23%, respectively.

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Abstract

The invention discloses a method for quickly detecting zearalenone toxin in vegetable oil. The method comprises the steps of uniformly mixing the vegetable oil and a methanol solution, centrifuging or carrying out settling separation after reaction, getting a supernatant, and utilizing a zearalenone toxin test strip for detecting. According to the detection method provided by the invention, the physicochemical property that zearalenone is easy to dissolve in organic solvents such as acetonitrile, methyl alcohol, ethyl alcohol, ethyl acetate and acetone is utilized, so that the method for simply and conveniently detecting zearalenone toxin in the vegetable oil is invented, and the development of oil and vegetable oil industries is promoted. The tests show that the method provided by the invention can be used for quickly detecting the zearalenone toxin in the vegetable oil, is high in efficiency, less in consuming time, low in cost and high in recovery rate, and meets the laboratory quality control standard-food physical and chemical detection standard.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a detection method of zearalenone toxin. Background technique [0002] Zearalenone (Zearaleuoue, ZEN), also known as F-2 toxin, is a non-steroidal mycotoxin mainly produced by Fusarium graminearum (Fusarium gramiuearum) and yellow Fusarium (F. culmorum). ZEN has strong reproductive toxicity and teratogenic effects. Zearalenone was first isolated from scab corn. It is widely found in moldy corn, sorghum, wheat, oats, barley and other grains and dairy products. It is the most widely polluted Fusarium toxin in the world. After zearalenone contaminates feed and food, it not only seriously affects the agricultural economy, but also brings serious food safety problems and threatens human health. Due to the harm of zearalenone to human body and animals, many countries have established the limit standard of mitaralenone in food. Australia stipulates that the mass fractio...

Claims

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Application Information

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IPC IPC(8): G01N21/75G01N30/02
CPCG01N21/75G01N30/02
Inventor 刘阳关绚丽
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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