Method for detecting glucose based on prussian blue membrane biological electrode

Abstract

The invention discloses a method for detecting glucose based on a prussian blue membrane biological electrode. The method comprises the following concrete steps: SS1) cleaning a sample tank; SS2) activating the enzyme reacting with the glucose on the prussian blue membrane biological electrode and cleaning the sample tank; SS3) performing high spot calibration and cleaning the sample tank; SS4) adding quantitative glucose solution in known concentration into the sample tank, using a detection circuit for detecting, judging as successful high spot calibration if the detected concentration scope of the glucose solution is within 95%-105% of the known concentration after the current value of the detection circuit is stable, cleaning the sample tank according to the process in the step SS1) and skipping to the step SS6, and if not, judging if the detection times exceeds three, if not, skipping to the step SS3 and replacing the prussian blue membrane biological electrode and cleaning the sample tank; SS5) performing low spot calibration; and SS6) adding quantitative unknown glucose solution into the sample tank and regarding a display concentration of the detection circuit as the concentration of the to-be-detected glucose solution after the current value of the detection circuit is stable.

Description

technical field [0001] The invention relates to a glucose detection method based on a Prussian blue membrane bioelectrode, which belongs to the technical field of fermentation industry. Background technique [0002] Glucose is the main carbon source of many fermentation products. The regulation of glucose must be in line with the metabolic characteristics of the strains to prevent the occurrence of situations where the concentration of glucose is too high or too low, that is, its concentration in the fermentation broth must be strictly controlled. In the early stage of fermentation, if the glucose concentration is too high, it will easily inhibit, inhibit and limit the growth of bacteria; in the later stage of fermentation, if the glucose concentration is too low, it will limit the growth of bacteria and product synthesis. Therefore, at present, the process feed glucose is often used in the industry to assist in the online detection of glucose concentration, so as to achieve...

AI Technical Summary

Problems solved by technology

[0003] The existing similar technology is to prepare an enzyme ink for glucose detection based on the unstable enzyme ink used in the glucose electrochemical biosensor based on electron transfer and the detection sensitivity defect. Its method is to add different concentrations of glucose to the detection The device is measured by linear voltammetry, and the relationship diagram of the current of different concentrations of glucose solutions with the potential change is obtained, and the concentration of the glucose solution can be obtained by combining the reciprocal of the polarization resistance and the standard curve between the glucose concentration.

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