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FSC (forward scatter) receiving system of flow cytometer

A flow cytometer and receiving system technology, applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of lack of low-noise flow cytometer FSC receiving system, etc., and achieve simple system, reasonable design, convenient and reliable use Effect

Inactive Publication Date: 2017-10-24
上海厦泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, in the prior art, there is still a lack of a better low-noise, wide-range flow cytometer FSC receiving system

Method used

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  • FSC (forward scatter) receiving system of flow cytometer
  • FSC (forward scatter) receiving system of flow cytometer
  • FSC (forward scatter) receiving system of flow cytometer

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Experimental program
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Embodiment Construction

[0030] Such as image 3 The block diagram of the FSC receiving system structure of a kind of flow cytometer provided by the invention and Figure 5 As shown in the circuit diagram of the FSC receiving system of a kind of flow cytometer provided by the invention:

[0031] The FSC receiving system of this flow cytometer includes a photoelectric sensor D3 and a transimpedance amplifier U2 connected to the output terminal of the photoelectric sensor D3, and also includes a controllable gain amplifier U3, a DC feedback circuit and a differential operational amplifier. The controllable gain amplifier U3 is connected to the output terminal of the transimpedance amplifier U2, and the input terminal and output terminal of the DC feedback circuit are respectively connected to the output terminal of the controllable gain amplifier U3 and the input terminal of the transimpedance amplifier U2; the output terminal of the controllable gain amplifier is connected to the differential operation...

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Abstract

The invention relates to an FSC (forward scatter) receiving system of a flow cytometer. The system comprises a photoelectric sensor, a transimpedance amplifier connected with an output end of the photoelectric sensor, a controllable gain amplifier, a DC (direct current) feedback circuit and a differential operational amplifier, wherein an input end of the controllable gain amplifier is connected with an output end of the transimpedance amplifier, and input and output ends of the DC feedback circuit are connected with an output end of the controllable gain amplifier and an input end of the transresistance amplifier respectively; an output end of the controllable gain amplifier is connected with an input end of the differential operational amplifier. The FSC receiving system of the flow cytometer has the characteristics of low noise and wide range, total noise of the system is 115.12 mu V, adjustable gain is realized in the range of 10 dB-30 dB, and the system can adapt to accurate detection of cells of different sizes being 0.2-20 mu m and meets demands of scientific research; the system is reasonable in design, simple and convenient and reliable to use, and has great application and popularization value.

Description

technical field [0001] The invention relates to a flow cytometer, in particular to an FSC receiving system of the flow cytometer, and belongs to the technical field of biomedical detection devices. Background technique [0002] Flow cytometry generally includes: an optical system, a liquid flow system, a detection and data processing system, and some flow cytometers also include a cell sorting system, which is a fast, accurate, objective, and simultaneous detection High-tech biomedical detection instruments with multiple physical and biological characteristics of single cells are widely used in the research and quantitative research of biological cell characteristics. [0003] Such as figure 1 - The schematic diagram of the optical path of the FSC receiving system is shown: when the cells intersect the laser beam in the liquid column, they scatter light to the surrounding 360° solid angle direction. The intensity of the scattered light is related to the size, shape, and ref...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14
CPCG01N15/1429
Inventor 顾秋涛
Owner 上海厦泰生物科技有限公司
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