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Application of compound epothilone b in preparation of medicine for repairing corneal nerve trauma

A technology of epothilone and nerve trauma, applied in the field of biomedicine, can solve the problems of accelerating corneal nerve trauma repair and functional recovery, achieve important development and application prospects, inhibit depolymerization, and increase concentration

Active Publication Date: 2020-09-04
广州昇藤教育科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant research reports on the use of EpoB to accelerate corneal nerve trauma repair and functional recovery

Method used

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  • Application of compound epothilone b in preparation of medicine for repairing corneal nerve trauma
  • Application of compound epothilone b in preparation of medicine for repairing corneal nerve trauma
  • Application of compound epothilone b in preparation of medicine for repairing corneal nerve trauma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. EpoB accelerates the repair of corneal nerve trauma in mice

[0025] 1. Laboratory animals

[0026] 8-week-old C57 / BL6 female mice, weighing 15-20g, were purchased from Guangdong Experimental Animal Center. Before the experiment, 24 mice checked the eyes of the animals to ensure that the experimental mice had no eye diseases, corneal defects and conjunctival damage.

[0027] 2. Experimental drugs

[0028] Dissolve EpoB in DMSO (dimethyl sulfoxide) to prepare a solution with a concentration of 5 mg / mL, and set aside. EpoB stock solution was diluted with normal saline at 1:200, mice were injected intraperitoneally with 0.1mg / mL EpoB, and mice in the control group were injected intraperitoneally with the same amount of blank solvent.

[0029] 3. Experimental method

[0030] Twenty normal mice without eye diseases were selected and randomly divided into trauma control group and EpoB administration group. After each group of mice were given corresponding administration op...

Embodiment 2

[0033] Example 2. EpoB accelerates the recovery of injured corneal nerve function in rats

[0034] 1. Laboratory animals

[0035] Thirty healthy male SD rats of 8 weeks old, weighing about 250±20g, were purchased from Guangdong Experimental Animal Center. The eyes of the animals were examined within 24 hours before the experiment. Animals without eye irritation, corneal defects or conjunctival damage were used in the experiment.

[0036] 2. Experimental drugs

[0037] Dissolve SR8278 (purchased from sigma, USA) in an appropriate amount of DMSO, add soybean oil for injection, and prepare SR8278 oily eye drops with a drug content of 0.2%.

[0038] 3. Establishment of rat corneal nerve injury animal model

[0039] Rats were anesthetized with Sumianxin (purchased from Jilin Province Huamu Animal Health Products Co., Ltd.), and a cored corneal trephine with a diameter of 3mm was used under a microscope to drill a ring with a diameter of 3mm and a depth of 0.5mm in the center of the cornea. ...

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Abstract

The invention discloses application of a compound epothilone B to preparing a medicine for healing cornea neurotrauma, and belongs to the field of biological medicines. The pharmacodynamic experiment proves that a rat suffering from cornea neurotrauma is taken as an experimental subject and the area of a cornea nerve is obviously increased by EpoB and is close to the normal level. The compound EpoB stabilizes the tubulin binding, so that the tubulin polymerization is promoted, the depolymerization of a microtube is inhibited, the concentration of the tubulin is increased, the repairing and the reconstruction of the neurotrauma are promoted, and the normal structure and functions of the cornea are maintained. The EpoB has better treatment effects on the function injuries and the cornea nerve lesion caused by refractive surgery, keratoplasty, herpes simplex keratitis, xerophthalmia, diabetes and long-term use of eyedrops, and alleviates the eye symptoms of a patient; and the medicine is low in toxicity and high in stability, and has an important development and application prospect.

Description

Technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of a compound Epothilone B (EpoB) in preparation of a medicine for repairing corneal nerve trauma. Background technique [0002] Since Sohlemm discovered the corneal nerve in the limbus in 1830, the research of corneal nerve has made great progress. The corneal tissue has no blood vessels, but is rich in nerve fibers. It is one of the most densely nerved tissues in the human body. It plays an important role in providing nutrition and metabolism and maintaining the normal structure and function of the cornea. The corneal sensation is the most prominent feature of the corneal nerve, which has a keen sensory function. Corneal nerves are peripheral nerves and have incomplete regeneration ability. Although corneal nerves can slowly regenerate after trauma, it is difficult to restore normal nerve density and function. [0003] Surgery injuries, eye diseases, and long-te...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/427A61P25/02A61P27/02
CPCA61K31/427
Inventor 李志杰董栋王寒晴肖程聚薛芸霞
Owner 广州昇藤教育科技有限公司
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