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CAPS marker detection method and primer relevant to soybean cyst nematode resistance

A soybean cyst nematode, soybean technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of difficult success, inability to deal with nematode toxicity variation, long time, etc., to avoid time-consuming and labor-intensive, The breeding effect is remarkable and the effect of improving the selection efficiency

Active Publication Date: 2017-10-27
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

Due to the genetic complexity of SCN resistance genes and the diversity of soybean cyst nematode physiological races, it takes a long time to breed soybean cyst nematode-resistant varieties using traditional breeding methods, and it is difficult to succeed and cannot cope with nematodes Toxic variation of SCN cannot satisfy the current development of line-resistant breeding, and a more accurate and efficient method for identifying SCN is urgently needed

Method used

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  • CAPS marker detection method and primer relevant to soybean cyst nematode resistance
  • CAPS marker detection method and primer relevant to soybean cyst nematode resistance
  • CAPS marker detection method and primer relevant to soybean cyst nematode resistance

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Embodiment 1

[0032] Example 1. Development and application of CAPS markers related to soybean cyst nematode resistance

[0033] 1. Primer design and marker development

[0034] Download the Glyma.11G234500 gene sequence from phytozome (https: / / phytozome.jgi.doe.gov / pz / portal.html), the gene Glyma.11G234500 and its homolog Glyma.18G022500 have high nucleotide sequences Therefore, in order to ensure the specificity of the primer sequences, the sequence alignment of the two genes is performed first, and primers are designed at the difference sites. Primer pair A was designed using Primer5.0 software. Utilize the online software dCAPSFinder2.0 (http: / / helix.wustl.edu / dcaps / dcaps.html) to develop the SNP as a CAPS marker (the marker site information selected in the present invention is shown in Table 1), select the enzyme AciI ( Beijing Bailingke Biological Co., Ltd., product number: R0551S) for enzyme digestion analysis. The PCR amplification product of primer pair A is expected to be 283 b...

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Abstract

The invention discloses a CAPS marker detection method and a primer relevant to soybean cyst nematode resistance. The invention provides application of single nucleotide polymorphism of following SNP locus in a soybean genome or a substance for detecting the single nucleotide polymorphism of the following SNP locus in the soybean genome for identifying or assisting identification of soybean resistance to the soybean cyst nematode; the SNP locus in the soybean genome corresponds to 113th site of nucleotide sequence shown as sequence 1 in a sequence list; and nucleotide at the SNP locus is G or T. The CAPS marker detection method and the primer relevant to the soybean cyst nematode resistance can select materials resistant to the soybean cyst nematode by detecting genotype of the SNP locus, can avoid time-consuming and labor-consuming phenotypic identification, can carry out soybean cyst nematode resistance screening on breeding materials through the genotype, increase selection efficiency of resistant varieties in a breeding process and shorten the breeding time largely, and improve cultivation effects of varieties resisting the soybean cyst nematode significantly.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a CAPS marker detection method and primers related to soybean cyst nematode resistance. Background technique [0002] Soybean Cyst Nematode (SCN) is an essential static parasitic nematode in roots, which causes huge losses to soybean production. In the United States alone, from 1996 to 2014, soybean cyst nematode caused about 36% of the damage caused by various diseases on soybeans. Breeding resistant material and incorporating crop rotation is a major method of disease control. Due to the genetic complexity of the SCN resistance gene and the diversity of the physiological races of soybean cyst nematode, using traditional breeding methods to develop soybean cyst nematode-resistant varieties not only takes a long time, but also is difficult to succeed and cannot deal with nematodes. Therefore, a more accurate and efficient method for identifying SCN is urgently needed. With the devel...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 邱丽娟李英慧田宇
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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