Anti-soybean cyst nematode-related caps marker detection method and primers
A soybean cyst nematode and soybean technology, applied in the biological field, can solve problems such as difficulty in success, inability to cope with nematode toxicity variation, long time, etc., and achieve the effects of avoiding time-consuming and labor-intensive, remarkable cultivation effect, and improving selection efficiency.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] Example 1. Development and application of CAPS markers related to soybean cyst nematode resistance
[0033] 1. Primer design and marker development
[0034] Download the Glyma.11G234500 gene sequence from phytozome (https: / / phytozome.jgi.doe.gov / pz / portal.html), the gene Glyma.11G234500 and its homologous gene Glyma.18G022500 have a high nucleotide sequence Therefore, in order to ensure the specificity of the primer sequence, the sequence comparison of the two genes was carried out first, and the primers were designed at the difference sites. Primer pair A was designed using Primer5.0 software. Use the online software dCAPSFinder2.0 (http: / / helix.wustl.edu / dcaps / dcaps.html) to develop SNPs into CAPS markers (the marker site information selected by the present invention is shown in Table 1), select the enzyme AciI ( Beijing Bailingke Biotechnology Co., Ltd., Cat. No.: R0551S) for enzyme digestion analysis. The expected length of the PCR amplification product of primer...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com