Anti-soybean cyst nematode-related caps marker detection method and primers

A soybean cyst nematode and soybean technology, applied in the biological field, can solve problems such as difficulty in success, inability to cope with nematode toxicity variation, long time, etc., and achieve the effects of avoiding time-consuming and labor-intensive, remarkable cultivation effect, and improving selection efficiency.
CN107299146BActive Publication Date: 2020-07-14INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Publication Date
2020-07-14

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Abstract

The invention discloses a CAPS marker detection method and a primer relevant to soybean cyst nematode resistance. The invention provides application of single nucleotide polymorphism of following SNP locus in a soybean genome or a substance for detecting the single nucleotide polymorphism of the following SNP locus in the soybean genome for identifying or assisting identification of soybean resistance to the soybean cyst nematode; the SNP locus in the soybean genome corresponds to 113th site of nucleotide sequence shown as sequence 1 in a sequence list; and nucleotide at the SNP locus is G or T. The CAPS marker detection method and the primer relevant to the soybean cyst nematode resistance can select materials resistant to the soybean cyst nematode by detecting genotype of the SNP locus, can avoid time-consuming and labor-consuming phenotypic identification, can carry out soybean cyst nematode resistance screening on breeding materials through the genotype, increase selection efficiency of resistant varieties in a breeding process and shorten the breeding time largely, and improve cultivation effects of varieties resisting the soybean cyst nematode significantly.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a method for detecting a CAPS marker related to soybean cyst nematode resistance and primers. Background technique

[0002] Soybean Cyst Nematode (SCN) is an essential static parasitic nematode in roots, which causes huge losses to soybean production. In the United States alone, from 1996 to 2014, soybean cyst nematodes accounted for approximately 36% of the damage caused by various diseases on soybeans. Breeding resistant materials and incorporating crop rotations is a major approach to disease control. Due to the genetic complexity of SCN resistance genes and the diversity of soybean cyst nematode physiological races, it takes a long time to breed soybean cyst nematode-resistant varieties using traditional breeding methods, and it is difficult to succeed and cannot cope with nematodes The toxic variation of SCN cannot satisfy the current development of line-resistant breeding, and ...

Claims

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