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A kind of artificial sequence of American cockroach peptide a with anti-liver cancer activity and its application

A technology of artificial sequence and American cockroach, which is applied in the field of medicine, can solve the problems of hypersensitivity, limited research on active substances, and failure to meet the needs of the pharmaceutical market, and achieve the effect of no elimination reaction and small dosage

Active Publication Date: 2020-03-27
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These extracts have a great impact on the further preparation of anticancer drugs. For example, impurities in the extracts are likely to cause hypersensitivity, which limits the way of drug use. At the same time, further separation and purification will greatly reduce the efficacy of the drug
Therefore, there are not many studies on the active substances of single components in the American cockroach at present, which is far from meeting the needs of the pharmaceutical market.

Method used

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  • A kind of artificial sequence of American cockroach peptide a with anti-liver cancer activity and its application
  • A kind of artificial sequence of American cockroach peptide a with anti-liver cancer activity and its application
  • A kind of artificial sequence of American cockroach peptide a with anti-liver cancer activity and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The artificial sequence of American cockroach peptide A is prepared on the basis of American cockroach peptide A through artificial transformation. American cockroach peptide A is present in the medicinal materials of Periplaneta americana. The extraction method of the American cockroach peptide A comprises the following steps: after extracting the Periplaneta americana medicinal material with a solvent, and then repeatedly extracting it by reverse phase column chromatography, dextran gel column chromatography, gel electrophoresis and in-gel recovery, etc. After separation, a sample containing melitonin A was obtained.

[0039] The N-terminal 12 amino acid residue sequence "NH 2 -Thr-Val-Tyr-Ser-Arg-Cys-Gly-Leu-Val-Gln-Ala-Lys-COOH" is transformed on the basis of "Cys" at position 6 of the sequence into " Arg", the "Leu" at the 8th position was transformed into "Asp", and the "Ala" at the 11th position was deleted to form the artificial sequence of cockroach peptide A...

Embodiment 2

[0046] 1. Cell culture of human liver cancer HepG2 cell line

[0047] Human liver cancer HepG2 cell line adopts high-glucose DMEM complete medium containing 10% fetal bovine serum, placed in 5% CO 2 , Cultured in a 37°C incubator, and the cells in the logarithmic growth phase were taken for experiments.

[0048] 2. MTT method to detect the survival rate of human liver cancer cell HepG2 cells after treatment

[0049] HepG2 cell seed plate (1×10 5 mL -1 , 100 μL) were inoculated in a 96-well plate and cultured for 24 h. It is configured as an artificial sequence solution of American cockroach peptide A with a concentration of 200 μg / mL, and intervenes with the above-mentioned concentration of Periplaneta americana peptide solution. Each concentration has 6 replicate wells, and each hole is 200 μL. Thalidomide (concentration: 100 μg / mL, 200 μg / mL) positive control group and blank control group, continue to culture for 24 hours. The absorbance value A of each well was detecte...

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Abstract

The invention provides a periplaneta americana L. peptide A artificial sequence with anti-hepatoma activity and the application thereof, and belongs to the technical field of medicines. The periplaneta americana L. peptide A artificial sequence with the anti-hepatoma activity has an amino acid sequence as shown in SEQ ID No.1. The invention further provides the application of the periplaneta americana L. peptide A artificial sequence in preparation of a medicine for preventing and / or treating liver cancer. The periplaneta americana L. peptide A artificial sequence can effectively inhibit cell proliferation and migration of hepatoma cells HepG2 and inhibit increase of the intensity along with increase of the concentration of the periplaneta americana L. peptide A artificial sequence. Compared with a periplaneta americana L. extract, the periplaneta americana L. peptide A artificial sequence has an obvious advantage of the HepG2 proliferation inhibition effect; compared with thalidomide positive control, the periplaneta americana L. peptide A artificial sequence has the same or similar anti-hepatoma effect. The periplaneta americana L. peptide A artificial sequence is a polypeptide which can be conveniently and economically produced in a mass in an artificial synthesis way, so that limitation of resources of active substances is eliminated, and market and production demands can be met.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an artificial sequence of American cockroach peptide A with anti-liver cancer activity and its application. Background technique [0002] At present, due to environmental pollution and other problems brought about by industrial development, the quality of human living environment is continuously declining, and the incidence and mortality of tumor diseases are also increasing, which seriously threatens people's health and life. The occurrence of cancer is affected by many reasons, such as environmental pollution, chemical pollution, ionizing radiation, free radical toxins or microorganisms and their metabolic toxins and other carcinogens, and may also be affected by the body itself, such as genetic characteristics, endocrine imbalance, immune Functional disorders, etc. can also cause normal cells to become cancerous. According to data released by the International Un...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435A61K38/08A61P35/00A61P35/04
CPCA61K38/00C07K14/43563
Inventor 张成桂罗廷顺彭芳袁发璐李婷张蕊李玥杨志斌
Owner DALI UNIV
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