Collection device and collection method of mesenchymal stem cells

A mesenchymal stem cell and collection device technology, applied in the biological field, can solve problems such as difficulties in collecting mesenchymal stem cells, achieve the effects of avoiding cell loss, avoiding chemical and biological pollution, and improving the biological effect of materials

Active Publication Date: 2020-05-19
卢建熙 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] In view of this, the object of the present invention is to provide a collection device and collection method of mesenchymal stem cells, to solve the problem of difficult collection of mesenchymal stem cells

Method used

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  • Collection device and collection method of mesenchymal stem cells
  • Collection device and collection method of mesenchymal stem cells
  • Collection device and collection method of mesenchymal stem cells

Examples

Experimental program
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Effect test

Embodiment 1

[0049] refer to Figure 3-4 , this embodiment provides a collection device for mesenchymal stem cells, comprising:

[0050] The liquid storage device 10 is used to contain blood and / or bone marrow fluid. The liquid storage device 10 includes: a liquid storage cavity 11 and a first pusher 12, the liquid storage cavity 11 has a liquid storage cavity, and the first pusher One end of 12 is in sealing fit with the inner wall of the liquid storage chamber, and can slide along the inner wall of the liquid storage chamber to push the liquid in the liquid storage chamber;

[0051] The storage device 20 is used to accommodate porous bioceramics (i.e. the filter body). The storage device 20 includes: a storage cavity 21, a spacer cavity 22 and a second propeller 23. The storage cavity 21 has a storage Cavity, the spacer cavity 22 has a spacer cavity, the outer wall of the spacer cavity 22 is sealed and fitted with the inner wall of the storage cavity, and can slide along the inner wall ...

Embodiment 2

[0061] Based on the collection device for mesenchymal stem cells provided in Implementation 1, this embodiment provides a method for collecting mesenchymal stem cells, see Figure 5 , the method for collecting mesenchymal stem cells comprises the following steps:

[0062] S1, loading the porous bioceramic into the storage cavity of the storage cavity under aseptic conditions;

[0063] S2, inject blood and / or bone marrow fluid into the liquid storage cavity through the three-way valve;

[0064] S3, pushing the first propeller, so that blood and / or bone marrow fluid enters the spacer cavity through the porous bioceramic;

[0065] S4, push the second propeller, so that the blood and / or bone marrow fluid enters the liquid storage cavity through the porous bioceramic again;

[0066] S5, after repeating steps S3 and S4 several times, take out the porous bioceramic for use.

[0067] The collected mesenchymal stem cells can be eluted for in vitro culture or composite materials for ...

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Abstract

The invention relates to a collection device and collection method for mesenchymal stem cells. The collection device for mesenchymal stem cells includes a liquid storage apparatus and a material storage apparatus that are communicated with each other, the liquid storage apparatus is used for accommodating blood and / or bone marrow fluid, the material storage apparatus is used for accommodating porous bioceramics. The blood and / or bone marrow fluid flows between the liquid storage apparatus and the material storage apparatus to and fro, and are / is filtered by the porous bioceramics in the material storage apparatus, so that mesenchymal stem cells in blood and / or bone marrow fluid remain on the porous bioceramics. The collection device and collection method provided by the invention are carried out in an aseptic environment, can avoid the probability of chemical and biological pollution, and the compounded product can be used in clinical practice immediately. The method and device provided by the invention can be used in the fields of biology, pharmacy, medicine and veterinary medicine, etc.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a collection device and collection method of mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells are an important member of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development, and belong to pluripotent stem cells. Mesenchymal stem cells can now be isolated from various tissues, including periosteum, cancellous bone, fat , skeletal muscle, bone marrow fluid and blood. It has attracted increasing attention because of its multi-directional differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. Mesenchymal stem cells also have the ability to differentiate into muscle cells, liver cells, osteoblasts, chondrocytes, stromal cells and other cells under specific induction conditions in vivo or in vitro, and can also be used as ideal seed cells for a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/26C12M1/12C12N5/0775
CPCC12M33/14C12M47/02C12N5/0663C12N5/0665
Inventor 卢建熙卢霄王臻
Owner 卢建熙
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