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A method and device for realizing the spontaneous sequential flow of multiple liquids

A liquid and sequential technology, applied in the field of microfluidics, can solve the problems of occupying work time and affecting work efficiency, and achieve the effect of improving work efficiency and saving labor time.

Active Publication Date: 2019-05-03
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the above-mentioned purification operation needs to manually add different processing reagents to the sample one at a time. The repeated reagent addition operation takes up a lot of working time and seriously affects the work efficiency.

Method used

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  • A method and device for realizing the spontaneous sequential flow of multiple liquids
  • A method and device for realizing the spontaneous sequential flow of multiple liquids
  • A method and device for realizing the spontaneous sequential flow of multiple liquids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] In Embodiment 1, the pressurizer 1 and the airtight container 3 are syringes, specifically, a 20ml syringe is used as the pressurizer 1, three 5ml syringes are used as the airtight container 3, and the three airtight containers 3 are connected to the air supply pipe 2 and the airtight container 3. The compressor 1 is connected, and the gas filling pipe 2 has an air inlet valve 21 near the airtight container 3 . Fix the three airtight containers 3 to the positions of 1ml, 3ml and 5ml respectively, and inject 100 microliters of blue ink a, red ink b and green ink c into the three airtight containers 3 respectively. The end of airtight container 3 links to each other with the connecting pipe 4 that flow path volume is all equal, and three connecting pipes 4 are connected with micropipe 5 by four-way pipe, and the end of micropipe 5 is connected with exhaust valve (not marked among the figures).

[0035] After completing the assembly of the device, close the exhaust valve a...

Embodiment 2

[0037] In the second embodiment, the pressurizer 1 and the airtight container 3 are syringes, specifically, a 20ml syringe is used as the pressurizer 1, and three 5ml syringes are used as the airtight container 3, and the three airtight containers 3 are connected to the air supply pipe 2 and the airtight container 3. The compressor 1 is connected, and the gas filling pipe 2 has an air inlet valve 21 near the airtight container 3 . Fix the three airtight containers 3 to the positions of 2ml, and inject 100 microliters of blue ink a, red ink b and green ink c into the three airtight containers 3 respectively. The end of the airtight container 3 is connected to the connecting pipe 4 with different flow channel volumes, wherein the blue ink a corresponds to the connecting pipe 4 with a length of 10 cm, the red ink b corresponds to the connecting pipe 4 with a length of 20 cm, and the green ink c corresponds to the connecting pipe 4. The length of pipe 4 is 40cm, and three connecti...

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Abstract

The invention provides a method and device for realizing the spontaneous sequential flow of multiple liquids. The method is characterized in that, firstly, the system composed of the airtight container (3) and the connecting pipe (4) is inflated and pressurized to make the internal pressure of the system greater than the external pressure, and then intercept a part of the high-pressure gas through the liquid blockage, and finally connect the connecting end of the connecting pipe (4) to the outside of the system, so that the airtight container (3) and the connecting pipe (4) A pressure difference is formed between them, so the liquid flows spontaneously under the action of the high-pressure gas, and when the volumes of the airtight containers (3) are different, the volumes of the flow channels of the connecting pipes (4) are all equal, or the volumes of the airtight containers (3) are the same. At the same time, the flow channel volumes of the connecting pipes (4) are not equal, so the time for each part of the liquid to reach the connecting end of the connecting pipe (4) is different, thereby realizing the sequential flow of multiple parts of liquid.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a method and device for realizing the spontaneous sequential flow of multiple liquids. Background technique [0002] In the field of biology and chemistry, the operation of purifying a certain substance is often encountered. During the purification, various liquids (processing reagents) are often required to process the samples to be purified in sequence. At present, the above-mentioned purification operation requires manual addition of different treatment reagents to the sample one at a time, and repeated reagent addition operations take up a lot of working time and seriously affect work efficiency. [0003] Therefore, how to realize the sequential addition of various reagents to the sample through simpler operations, so as to save labor time and improve work efficiency, has become a technical problem to be solved urgently by those skilled in the art. Contents of the inv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/10
CPCG01N35/10G01N2035/1044
Inventor 吴文明穆全全李渊明
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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