Retinal photoreceptor cell specificity surface protein screening method

Abstract

The invention relates to a retinal photoreceptor cell specificity surface protein screening method. The photoreceptor cell specificity surface protein can be screened out, specificity of the surface protein is specifically expressed on the surfaces of retinal photoreceptor cell membranes sourced from human embryonic stem cells and human multipotential stem cells, and a simple method is provided for purifying retinal photoreceptor cells source from the human multipotential stem cells. The purified photoreceptor cells obtained by screening the surface protein can avoid the potential danger caused by transgenosis or utilizing varieties of small molecule compound as inducers. As the key scientific problem is solved, a process for converting the stem cells to clinic is quickened, medical research need is met, and foundation of cell replacement therapy on human retinal related diseases (such as glaucoma, macula lutea eye disease and the like is established.

Description

technical field [0001] The invention relates to the field of biomedicine, and relates to retinal cells, in particular to a screening method for specific surface proteins of retinal photoreceptor cells. Background technique [0002] The retina is a key structure that makes up the visual system. Clinically, there are effective treatments for refractive visual dysfunction, but there is still no solution for retinopathy, especially visual impairment or blindness caused by trauma, degeneration and other reasons. Since the retina is derived from the neuroectoderm, it has typical structural characteristics of the central nervous system, is sensitive to damage, and is difficult to regenerate, making it the crux of refractory eye diseases, and it is also the focus, difficulty, and area that needs to be overcome urgently in ophthalmology research. Over the years, many researchers at home and abroad have carried out extensive explorations for this purpose, and the Ministry of Science ...

AI Technical Summary

Problems solved by technology

The reasons are obvious. Firstly, when the differentiated cells are used for drug screening or functional identification in vitro, the reliability of the results will be affected by the mixing of other cells; secondly, if the mixed cells are transplanted into model animals, abnormalities will occur. Risk of hyperplasia or even tumorigenesis

Although many studies have been devoted to improving the induction and differentiation rate of ganglion cells, for example: transfecting Math5 into stem cells, and then obtaining purified ganglion cells through FACS screening, however, these methods have a fatal flaw, that is: transfecting the reporter vector Infection into stem cells will lead to changes in the stem cell genome, which limits its clinical application