Supercritical CO2 extraction method for flavonoids in Selaginella doederleinii Hieron

A technology of flavonoids and dark green selaginella, applied in organic chemistry, bulk chemical production, etc., can solve problems such as patents that have not been reported, and achieve the effects of reducing costs, reducing usage, and reducing waste

Inactive Publication Date: 2017-11-24
天津市泰通农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the process of extracting flavonoids from Selaginella selaginella by supercritical carbon dioxide extraction technology, and no patents have been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 230g of dark green Selaginella dry powder, crush it until the particle size is between 40 mesh and 80 mesh, add 700mL of 95% ethanol and stir evenly, put it in a closed container and soak it in a dark place away from light for 6 hours, then put it into 1L of supercritical fluid In the extraction tank, the extraction conditions are: the extraction pressure is 25Mpa, the temperature is 45°C; the separation conditions are: the pressure of the first separation tank is 5Mpa, the temperature is 45°C, the pressure of the second separation tank is 5Mpa, the temperature is 35°C, and the liquid carbon dioxide The flow rate is 30L / h, which is recycled in the process. After 2 hours of dynamic operation, 800 mL of dark green Selaginella flavonoids extract was obtained from the primary and secondary separation kettles, and the extraction rate of flavonoids in dark green Selaginella was 2.89%.

Embodiment 2

[0022] Take 250g of dark green Selaginella dry powder, crush it until the particle size is between 40 mesh and 80 mesh, add 750mL of 85% ethanol and stir evenly, put it in a closed container and soak it in a dark place away from light for 6 hours, then put it into 1L of supercritical fluid extraction In the tank, the extraction conditions are: extraction pressure is 30Mpa, temperature is 50°C; separation conditions are: the pressure of the first separation tank is 6Mpa, the temperature is 45°C, the pressure of the second separation tank is 5Mpa, the temperature is 40°C, and the flow rate of liquid carbon dioxide It is 30L / h, which is recycled in the process. After 2.5 hours of dynamic operation, 874 mL of dark green Selaginella flavonoids extract was obtained from the primary separation kettle and the secondary separation kettle. The extraction rate of flavonoids in Selaginella dark green is 2.93%.

Embodiment 3

[0024] Take 280g of dark green Selaginella dry powder, crush it until the particle size is between 40 mesh and 80 mesh, add 900mL of 80% ethanol and stir evenly, put it in a closed container and soak it in a dark place away from light for 6 hours, then put it into 1L of supercritical fluid extraction In the tank, the extraction conditions are: extraction pressure is 35Mpa, temperature is 55°C; separation conditions are: the pressure of the first separation tank is 7Mpa, the temperature is 50°C, the pressure of the second separation tank is 6Mpa, the temperature is 45°C, and the flow rate of liquid carbon dioxide It is 30L / h, which is recycled in the process. After dynamic operation for 3 hours, 1170 mL of dark green Selaginella flavonoids extract was obtained from the primary separation kettle and the secondary separation kettle. The extraction rate of flavonoids in Selaginella dark green is 3.01%.

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Abstract

The invention relates to a supercritical CO2 extraction method for flavonoids in Selaginella doederleinii Hieron. The method comprises the following steps: the Selaginella doederleinii Hieron is dried, ground and soaked, and extraction is performed at the pressure of 20-35 MPa and the temperature of 40-60 DEG C for 2-7 h with 60%-95% ethanol as an entrainer; primary reduced-pressure separation is performed in a separation kettle at the pressure of 5-10 MPa and the temperature of 40-60 DEG C; secondary reduced-pressure separation is performed at the pressure of 4-7 MPa and the temperature of 35-50 DEG C, and a product is obtained from the separation kettle of a supercritical CO2 extraction device. The supercritical CO2 extraction method has the characteristics that the extraction temperature is low, the speed is high, the extraction rate is high, the obtained product can effectively retain active flavonoid ingredients in the Selaginella doederleinii Hieron and the like.

Description

technical field [0001] The invention belongs to the technical field of preparation of flavonoids, and relates to a method for extracting flavonoids in Selaginella chinensis, in particular to a method for extracting flavonoids in Selaginella selaginella by supercritical carbon dioxide. Background technique [0002] Selaginella doederleinii Hieron is a plant of the genus Selaginella in the family Selaginellaceae. Widely used in some parts of southern my country, aliases: Rooting Selaginella, Shishang Cypress, Big Leafy Vegetables, Thoreau Grass, Di Thoreau, Golden Dragon Grass, Dragon Scale Grass, Arborvitae, Shrimp Hemp Leaf, Crust Grass, Yanqing, Rock cypress, passing centipede, big windtail grass, ground cypress grass. "Guangxi Standards of Chinese Medicinal Materials" records that the source of Selaginella selaginella is Selaginella Selaginella S.doederleinii; "Guizhou Folk Medicine" refers to Selaginella Selaginella Selaginella Selaginella Doederleinii, which has the effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40Y02P20/54
Inventor 赵樱洁
Owner 天津市泰通农业科技有限公司
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