Polypeptide for typing hemorrhagic fever with renal syndrome caused by Hantaan virus and Seroul virus

Abstract

The present invention discloses a polypeptide for typing hemorrhagic fever with renal syndrome caused by Hantaan virus and Seroul virus, wherein the polypeptide is selected from a polypeptide having an amino acid sequence represented by SEQ ID NO.1-SEQ ID NO.8. The invention further discloses a kit used for typing hemorrhagic fever with renal syndrome caused by Hantaan virus and Seroul virus and prepared by using the polypeptide as the effective component. According to the present invention, with the polypeptide, the detection of the clinical typing of the HTNV hemorrhagic fever with renal syndrome and the SEOV hemorrhagic fever with renal syndrome can be effectively achieved; and compared to the Western blot, the method of the present invention has the following advantages that the positive rate of the antibody in the patient serum is high, and the detection sensitivity is high.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a polypeptide used for typing hemorrhagic fever with renal syndrome caused by Hantaan virus and Seoul virus. Background technique [0002] Hemorrhagic fever with renal syndrome (HFRS) is a worldwide prevalent natural foci disease caused by Hantavirus. my country is the country most seriously affected by it in the world. The annual incidence of the disease accounts for more than 90% of the world's total. In the past 10 years, the annual incidence has remained at 40,000 to 60,000, which remains high and seriously endangers people's lives and health. In my country, hemorrhagic fever with renal syndrome is mainly caused by two types of viruses, Hantaan virus (HTNV) and Seoul virus (SEOV). HTNV and SEOV viruses have significantly different animal hosts, epidemic characteristics, and clinical disease severity. The symptoms of hemorrhagic fever with renal syndrome are much ...

AI Technical Summary

Problems solved by technology

[0003] Since the successful isolation of HTNV in the mid-1970s, the typing detection of this genus virus or serum samples has always relied on plaque reduction neutralization test (PRNT), but because HTNV grows slowly in in vitro passage cells, PRNT not only takes a long time It usually takes more than 10 days), and the requirements for experimental materials are relatively high, and it is difficult for ordinary medical units to carry out

[0004] In the mid-1990s, HTNV reverse transcription-polymerase chain reaction (RT-PCR), restriction fragment length polymorphism (RFLP) and sequencing typing technology were developed, but the sensitivity of the above methods is poor and the operation is complicated. The cost of the experiment is high, and it is not suitable for routine use in clinical laboratories and epidemiological sites

[0005] Although the hemagglutination inhibition test that has been widely used in China for many years has the advantages of simplicity and rapidity, when performing typing detection, the blood inhibition titers of serum samples of a considerable number of HTNV or SEOV virus-infected persons (or experimental animals) are very close, and it is difficult to accurate typing

[0006] In conclusion, the clinical symptoms of HFRS infected by HTNV and SEOV virus are similar, but the condition and outcome are different. The fatality rate of HTNV infection is high, while that of SEOV infection is low

At present, the typing of these two viruses in my country and the world is limited to laboratory research, and it is impossible to type the two diseases clinically, and it is impossible to make early predictions on the prognosis and treatment of patients

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