A sample pretreatment method for detecting fungicides in tea

A sample pretreatment and bactericide technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of large sample pretreatment error, low content of components to be tested, and influence of bactericide detection, etc., to achieve high accuracy, The effect of reducing the amount of solvent used and shortening the pretreatment time

Active Publication Date: 2020-10-13
安徽宏远职业卫生技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the wide variety of fungicides, complex components, various forms, low content of the components to be tested, and more ingredients in tea, which will affect the detection of fungicides, sample pretreatment generally takes a long time and involves many steps, resulting in The pretreatment error is large, so a new sample pretreatment method needs to be provided

Method used

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  • A sample pretreatment method for detecting fungicides in tea

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Effect test

Embodiment 1

[0019] A sample pretreatment method for detecting fungicides in tea leaves, comprising the steps of: pulverizing the tea leaves and mixing them with acetonitrile, ultrasonicating for 20 minutes, centrifuging at a speed of 6000r / min for 15 minutes to take the acetonitrile layer, washing the precipitate with water for 3 times, and washing each time The amount of water used for precipitation is the same, then centrifuge at a speed of 6000r / min for 15min to take the water layer, combine the acetonitrile layer and the water layer and filter to get the filtrate, wherein the weight-to-volume (g / ml) ratio of tea leaves and acetonitrile is 10:3, tea and water The weight-to-volume (g / ml) ratio of water is 10:1; add the adsorbent to the filtrate, shake for 35 minutes, centrifuge at a speed of 6000r / min for 8 minutes to take the adsorbent, and vortex with methanol for 5 times, each time for 2 minutes, The amount of methanol used for each vortex analysis is the same, and the analysis soluti...

Embodiment 2

[0022] A sample pretreatment method for detecting fungicides in tea leaves, comprising the steps of: pulverizing tea leaves and mixing them with acetonitrile, ultrasonicating for 30 minutes, centrifuging at a speed of 4000r / min for 20 minutes to take the acetonitrile layer, washing the precipitate with water twice, and washing each time The amount of water used for precipitation is the same, then centrifuge at a speed of 4000r / min for 20min to take the water layer, combine the acetonitrile layer and the water layer and filter to get the filtrate, wherein the weight-to-volume (g / ml) ratio of tea leaves and acetonitrile is 5:1, tea leaves and The weight-to-volume (g / ml) ratio of water is 20:3; add adsorbent to the filtrate, shake for 25min, centrifuge at 4000r / min for 12min to get the adsorbent, vortex analyze with methanol 4 times, analyze for 3min each time, The amount of methanol used for each vortex analysis is the same, and the analysis solution is combined, dried with nitro...

Embodiment 3

[0025] A sample pretreatment method for detecting fungicides in tea leaves, comprising the steps of: pulverizing tea leaves and mixing them with acetonitrile, ultrasonicating for 22 minutes, centrifuging at a speed of 5500r / min for 16 minutes to take the acetonitrile layer, washing the precipitate with water for 3 times, and washing each time The water consumption of the precipitation is the same, then centrifuge at a speed of 5500r / min for 16min to take the water layer, combine the acetonitrile layer and the water layer and filter to get the filtrate, wherein the weight-volume (g / ml) ratio of tea leaves and acetonitrile is 10:2.7, tea leaves and The weight-to-volume (g / ml) ratio of water is 20:2.3; add adsorbent to the filtrate, shake for 32min, centrifuge at 5500r / min for 9min to get the adsorbent, vortex with methanol for 5 times, each time for 2.2min , the amount of methanol used for each vortex analysis is the same, and the analysis solution is combined, dried with nitroge...

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Abstract

The invention discloses a sample pretreatment method for detecting fungicides in tea leaves, which comprises the following steps: pulverizing the tea leaves and mixing them with acetonitrile, ultrasonication, centrifugation to take the acetonitrile layer, washing the precipitate with water, centrifuging again to take the water layer, and merging the acetonitrile layer and the water layer And filter to get the filtrate; add adsorbent to the filtrate, oscillate, centrifuge to get the adsorbent, vortex analyze with methanol, take the analysis solution, blow dry with nitrogen, and then dissolve with methanol to obtain a sample solution. The invention has short pretreatment time, less solvent consumption, less steps and low error.

Description

technical field [0001] The invention relates to the technical field of sample pretreatment, in particular to a sample pretreatment method for detecting fungicides in tea leaves. Background technique [0002] China has a long history of tea culture, and tea leaves are found in almost every household. With the mass planting of tea leaves, it is inevitable to use fungicides to prevent diseases and kill insects, so as to increase production. However, with the use of fungicides, there will be fungicide residues on the tea leaves, which will endanger people's health. In order to monitor the fungicide residues in tea leaves, a variety of detection methods have emerged, such as high performance liquid chromatography, gas chromatography, etc., but all of them require pretreatment of tea leaves before detection. [0003] Due to the wide variety of fungicides, complex components, various forms, low content of the components to be tested, and more ingredients in tea, which will affect ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/14G01N30/08
CPCG01N30/06G01N30/08G01N30/14
Inventor 夏怀翔赵华芝孔玉萍严章飞方栋张强段志祥
Owner 安徽宏远职业卫生技术服务有限公司
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