Method and application for preparing amide and ester compounds by marine bacillus amyloliquefaciens

Abstract

A method for preparing 13-docosenamide by fermenting a marine bacillus amyloliquefaciens BMF01 bacterial strain comprises the following steps: fermentation of the BMF01 bacterial strain and preparation of fermentation broth thereof, acid precipitation of the fermented broth of the BMF01 bacterial strain and methanol extraction, silica gel column chromatography of BMF01 bacterial strain fermentation broth methanol extract, Sephadex LH-20 column chromatography, then the absorption peak of MD-4 components appear at 11.5 min, and is identified to be a structure of the compound 13-docosenamide. The invention also relates to a method for preparing ester compounds from marine bacillus amyloliquefaciens BMF01. The method of the invention produces the 13-docosenamide and ester compounds having the application of inhibiting root rot of wheat. For the first time, five kinds of compounds with bacteriostatic action are prepared on the basis of BMF01 strain. The method has the advantages of simple method and high operability.

Description

technical field [0001] The present invention relates to the method for extracting compound from the bacterial fermented liquor from ocean, especially from a kind of marine Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) A method for extracting antibacterial compounds from BMF01 strain fermentation broth. Background technique [0002] Bacillus amyloliquefaciens can produce a series of antibacterial substances during the growth process, such as enzymes, lipopeptides, β-1,3 glucanase, surfactant and iturin A, etc., which are effective against a variety of plant pathogens. preventive effect. Many scholars have used macroporous resin method, acid-base precipitation method, ammonium sulfate precipitation method, chromatography and other separation and extraction methods to study the metabolites of Bacillus amyloliquefaciens, and separated and purified a variety of substances that have inhibitory effects on plant pathogenic fungi. It is of great significance to understa...

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Problems solved by technology

Existing studies have shown that the types of antibacterial substances produced by different Bacillus amyloliquefaciens are different. At present, the strains related to the antibacterial substances of Bacillus amyloliquefaciens that have been studied more come from terrestrial sources. There are few identifications, and there is no report on the antibacterial activity of 13-carbadicoenamide isolated from the fermentation broth of Bacillus amyloliquefaciens from the sea and 13-carbadicoenamide

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