Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of anti-XRCC3 IgG antibody used as gastric cancer serum marker and kit of anti-XRCC3 IgG antibody

A diagnostic kit and serum technology, applied in the application of gastric cancer serum markers and the field of the kit, can solve the problem of inability to diagnose gastric cancer, and achieve the effects of easy operation, improved sensitivity and specificity

Active Publication Date: 2017-12-08
上海浦东解码生命科学研究院
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the defects in the sensitivity and specificity of the existing gastric cancer biomarkers. It is used for the diagnosis of gastric cancer. In order to realize the early diagnosis of gastric cancer with high sensitivity and high specificity, it provides an anti-XRCC3 IgG antibody as a serum marker for gastric cancer and its kit

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of anti-XRCC3 IgG antibody used as gastric cancer serum marker and kit of anti-XRCC3 IgG antibody
  • Application of anti-XRCC3 IgG antibody used as gastric cancer serum marker and kit of anti-XRCC3 IgG antibody
  • Application of anti-XRCC3 IgG antibody used as gastric cancer serum marker and kit of anti-XRCC3 IgG antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] 1. Preparation of serum samples:

[0024] Centrifuge the whole blood sample at 1000g for about 20 minutes after standing at room temperature for 2 hours or overnight at 4°C, and take the supernatant for immediate detection; or aliquot and store the sample at -20°C or -80°C, but avoid Freeze and thaw repeatedly. Thawed samples should be centrifuged again prior to testing. The tested sample cannot contain NaN3, because NaN3 inhibits the (HRP) activity of horseradish peroxidase.

[0025] 2. Preparation methods of various buffer solutions and reagents for ELISA method

[0026] Coating buffer: 0.05M Na at pH 9.6 2 CO 3 -NaHCO 3

[0027]

[0028] (2) Sample diluent: pH 7.4 PBS solution

[0029]

[0030] (3) Washing solution: PBST solution with pH 7.4

[0031]

[0032] (4) Blocking solution: pH 7.4 PBS solution of 0.5% BSA

[0033]

[0034]

[0035] (5) Enzyme substrate solution: chromogenic agent A and chromogenic agent B (both need to be prepared and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a reagent for detecting an anti-XRCC3 IgG antibody in a serum in preparing a kit for diagnosing and indicating a gastric cancer. The anti-XRCC3 IgG antibody is used as a gastric cancer serum marker, the level of the anti-protein XRCC3-IgG antibody in the human serum is qualitatively determined, the specificity of the gastric cancer is detected to be 87 percent, the sensitivity of the gastric cancer is 90 percent, the anti-XRCC3 IgG antibody can be used as a way of assisting early diagnosis of the gastric cancer, and the sensitivity and the specificity of early diagnosis of the gastric cancer are improved. The gastric cancer diagnosis kit prepared by utilizing the reagent for detecting the anti-XRCC3 IgG antibody in the serum is sensitive, safe, reliable and easy to operate.

Description

technical field [0001] The invention relates to a cancer marker, in particular to the application of an anti-XRCC3 IgG antibody as a gastric cancer serum marker and a kit thereof. Background technique [0002] Gastric cancer is a common malignant tumor that endangers human health. According to the statistics of the World Health Organization in 2008, the death rate of gastric cancer patients ranks second in the world. In 2006 the World Health Organization statistics showed nearly 950,000 new cases, while about 700,000 people died from this disease. In my country, the number of new gastric cancer patients exceeds 300,000 every year, and about 160,000 people die from gastric cancer. In order to reduce the mortality rate and improve the curative effect of gastric cancer, the key is the "three early" work of gastric cancer, that is, early detection, early diagnosis and early treatment. However, because the vast majority of gastric cancer patients lack specific clinical symptoms...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/535G01N33/536G01N33/574
CPCG01N33/535G01N33/536G01N33/57446
Inventor 李胜王靖方贺林陈培华秦胜营蒋太交宓现强李兴旺孟广勋张驰宇周育夫陈登宇
Owner 上海浦东解码生命科学研究院