A kind of method for rapid propagation of bamboo shoot orchid seedlings
A kind of seedling and rapid technology, applied in the field of rapid propagation of bamboo shoot orchid seedlings, can solve the problems that resources cannot meet the market demand, it is difficult to realize large-scale production, and the propagation speed of bamboo shoot orchid seedlings is slow, so as to achieve low material prices and high production efficiency. Low cost, uniform effect of seedlings
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Embodiment 1
[0055] A kind of method for rapid propagation of bamboo shoot orchid seedlings, the steps are as follows:
[0056] a. Select the stem tip of wild bamboo shoot orchid to obtain product A; the shoot tip of wild bamboo shoot orchid is selected from the tender part of the stem tip of the wild bamboo shoot orchid plant that has no yellow leaves and no disease in May to June;
[0057] b. Rinse product A with clean water, rinse twice with sterile water, each time for 10s, soak in 75% medical alcohol for 15s, wash with sterile water, and then use HgCl 2 The solution was sterilized twice, each time for 5 minutes, and the HgCl was washed with sterile water 2 , to obtain product B; the HgCl 2 The solution concentration is 0.1%;
[0058] c. Culture medium preparation: preparation of value-added medium C 1 rooting medium C 2 product, the value-proliferating medium C 1 The ingredients in the product include: MS+30g / L sucrose+6g / L agar+0.3g / L activated carbon powder+50g / L banana juice+1...
Embodiment 2
[0062] A kind of method for rapid propagation of bamboo shoot orchid seedlings, the steps are as follows:
[0063] a. Select the stem tip section of wild bamboo shoot orchid to obtain product A; the shoot tip section of the wild bamboo shoot orchid is selected from the tender part of the stem tip of the wild bamboo shoot orchid plant that has no yellow leaves and no disease in May to June;
[0064] b. Rinse product A with clean water, rinse 3 times with sterile water, each time for 15s, soak in 75% medical alcohol for 20s, wash with sterile water, and then use HgCl 2 The solution was sterilized twice, each time for 8 minutes, and the HgCl was washed with sterile water 2 , to obtain product B; the HgCl 2 The solution concentration is 0.15%;
[0065] c. Culture medium preparation: preparation of value-added medium C 1 rooting medium C 2 product, the value-proliferating medium C 1 The ingredients in the product include: MS+30g / L sucrose+6g / L agar+0.3g / L activated carbon powd...
Embodiment 3
[0069] A kind of method for rapid propagation of bamboo shoot orchid seedlings, the steps are as follows:
[0070] a. Select the stem tip section of wild bamboo shoot orchid to obtain product A; the shoot tip section of the wild bamboo shoot orchid is selected from the tender part of the stem tip of the wild bamboo shoot orchid plant that has no yellow leaves and no disease in May to June;
[0071] b. Rinse product A with clean water, rinse twice with sterile water, each time for 15s, soak in 75% medical alcohol for 18s, wash with sterile water, and then use HgCl 2 The solution was sterilized twice, 6 minutes each time, and the HgCl was washed with sterile water 2 , to obtain product B; the HgCl 2 The solution concentration is 0.13%;
[0072] c. Culture medium preparation: preparation of value-added medium C 1 rooting medium C 2 product, the value-proliferating medium C 1 The ingredients in the product include: MS+30g / L sucrose+6g / L agar+0.3g / L activated carbon powder+60g...
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