Method for Improving the Quality of Paper-making Tobacco Sheets Using Flavoring Composite Microbial Preparations
A technology of composite microorganisms and tobacco flakes, which is applied in the field of cigarette production technology, can solve problems such as obvious stimulation, low aroma, and adverse effects, and achieve the effects of improving sensory quality, convenient use, and increasing the content of aroma components in smoke
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Embodiment 1
[0022] Bacillus subtilis ( Bacillus subtilis ) Y1, Bacillus subtilis ( Bacillus subtilis ) X7 and Bacillus subtilis ( Bacillus subtilis ) Separation and screening of X8:
[0023] 1. Isolate the strains in the flavoring compound microbial preparation from the surface of natural alcoholized tobacco leaves, namely Bacillus subtilis ( Bacillus subtilis ) Y1, Bacillus subtilis ( Bacillus subtilis ) X7 and Bacillus subtilis ( Bacillus subtilis )X8, its separation method is: after first mixing 90mL sterile water with 10g alcoholized tobacco leaves (i.e. 10 -1 Suspension), use aseptic sampling method to take 1mL of the mixture and add it to 9mL sterile water to make 10 -2 suspension, and then serially diluted, take 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 Spread 0.2mL of suspensions of six dilutions on the improved LB medium, incubate at a constant temperature of 37°C for 24 hours, pick a single colony on a plate with good growth, full and uniform colonies, and appropriate ...
Embodiment 2
[0035] The bacillus subtilis that embodiment 1 obtains ( Bacillus subtilis ) Y1, Bacillus subtilis ( Bacillus subtilis ) X7, Bacillus subtilis ( Bacillus subtilis )X8 is compounded according to the mass ratio of 1:1:1 to obtain a fragrance-imparting microbial preparation, which is called preparation 1;
[0036] The bacillus subtilis that embodiment 1 obtains ( Bacillus subtilis ) Y1, Bacillus subtilis ( Bacillus subtilis ) X7, Bacillus subtilis ( Bacillus subtilis )X8 is compounded according to the mass ratio of 0.001:1:0.001 to obtain a fragrance-imparting microbial preparation, which is called preparation 2;
[0037] The bacillus subtilis that embodiment 1 obtains ( Bacillus subtilis ) Y1, Bacillus subtilis ( Bacillus subtilis ) X7, Bacillus subtilis ( Bacillus subtilis )X8 is compounded according to the mass ratio of 1:0.001:1 to obtain a fragrance-imparting microbial preparation, which is called preparation 3;
[0038] The bacillus subtilis that embodiment 1 obtains ...
Embodiment 3
[0042] The composite microbial preparation prepared in Example 2 is used for the fermentation of paper-making tobacco flakes, and the paper-making tobacco flakes are loosened and rehydrated and shredded to obtain paper-making tobacco flakes, according to 0.0001wt% of the paper-making tobacco flakes ~0.5wt% Weigh the compound microbial preparation prepared in Example 2, spray it on the paper-making tobacco flakes, and ferment at a constant temperature and humidity for 24h~ After 60 hours, the fermented paper-making tobacco flakes are dried until the moisture content of the tobacco flakes is between 10% and 15%, and the microbial preparation fermented tobacco flakes are obtained.
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