Monitoring system and method for dynamic characteristics of cells based on three-dimensional graphene interface electrode
A technology of interface electrodes and dynamic characteristics, applied in the field of microfluidic cell research, can solve the problems of inability to respond to cell micro-movement and migration, small contact area between electrodes and cells, unfavorable cell biological characteristics, etc., achieving good application prospects, transmission Effects of improved inductive performance and sensitivity, improved utilization and cost
Active Publication Date: 2017-12-15
ZHEJIANG SCI-TECH UNIV
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Problems solved by technology
At present, the sensing electrodes of most microfluidic chips are the classic flat two-dimensional gold electrode structure. The contact area between the electrode and the cells is small, the cell signal obtained is weak, and it cannot respond to the micro-movement of the cells in the vertical direction. and migration, so it is not conducive to the study of cell biology, especially single cells
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[0039] The present invention will be described in further detail below in conjunction with the accompanying drawings.
[0040] Such as figure 1 The construction diagram of all the devices of this system is shown, including the microfluidic chip fixed on the PCB, the syringe pump, the impedance meter and the current amplifier, as well as the microscope with CCD, each part is controlled by the computer.
[0041] The microfluidic chip consists of two liquid storage tanks 22 and a test flow channel 21 between the two liquid storage tanks 22 to form an H-shaped PDMS flow channel, and the PDMS cover that can be opened ( Figure 4 shaded part) and sub-chips arranged in the test channel, such as Figure 4 As shown; the design of the H-shaped PDMS flow channel can prevent the blockage of the chip when the cells are injected; the planar structure is as follows Figure 5 As shown, the tank wall of the liquid storage tank 22 or the cover plate that seals the liquid storage tank has open...
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The invention provides a monitoring system and method for dynamic characteristics of cells based on a three-dimensional graphene interface electrode, and specifically relates to parts including a microfluidic chip, a PCB (Printed Circuit Board), an impedance meter, an injection system, a current amplifier, an inverted microscope with a CCD (Charge Coupled Device), and the like. Cell impedance change of a single cell / two cells in a whole life cycle (including capturing, adhesion, migration and proliferation and differentiation) is acquired in real time, surface appearances of the cells are monitored in real time, and physiological characteristics corresponding to inner states of the cells and the surface appearances at different phases of cancerization, caused by a single cancer cell and a mutual effect between two cancer cells, are explored, so that a novel concept and a new research technology are provided for exploring cell biology. Compared with a traditional gold electrode, the single-cell sensing performance of the graphene electrode adopted by the system is averagely improved by 100 percent, and the double-cell sensing performance is averagely improved by 50 percent.
Description
technical field [0001] The invention belongs to the field of microfluidic cell research, and in particular relates to a cell dynamic characteristic monitoring system and method based on a three-dimensional graphene interface electrode. Background technique [0002] Cells are the basic structural and functional units of organisms, and cells are the most basic living systems. Traditional cell research usually takes a large number of cell samples as the object, expecting to obtain the general properties of the same kind of cells. However, there are also more or less differences between cells of the same type due to cell-to-cell interactions. This analysis effectively only provides the average response of the entire sample of cells, ignoring the details of individual cells. Therefore, it is crucial to design a microfluidic chip for the study of the sensing performance of single cells. [0003] Microfluidic chip technology integrates the basic operating units of sample prepara...
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IPC IPC(8): C12M1/00G01N15/10
CPCG01N15/10G01N33/5044G01N2015/1006
Inventor 陈本永刘爱萍王夏华吴化平邢赟许为中周奇
Owner ZHEJIANG SCI-TECH UNIV