Saccharum spontaneum L. drought stress expressed protein phosphatase 2C gene ScPP2C

A protein phosphatase, drought stress technology, applied in the field of biomolecule cloning, can solve problems such as the gene encoding 2C protein phosphatase has not been cloned

Inactive Publication Date: 2017-12-15
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the gene encoding 2C-type protein phosphatase and its functi

Method used

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  • Saccharum spontaneum L. drought stress expressed protein phosphatase 2C gene ScPP2C
  • Saccharum spontaneum L. drought stress expressed protein phosphatase 2C gene ScPP2C
  • Saccharum spontaneum L. drought stress expressed protein phosphatase 2C gene ScPP2C

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Embodiment 1

[0024] Example 1 Acquisition of 2C-type protein phosphatase gene ScPP2C expressed under drought stress in sugarcane thin stem wild species

[0025] 1. Design of primers to amplify the 2C protein phosphatase gene ScPP2C expressed in the wild sugarcane slender stem species under drought stress

[0026]According to the obtained 2C-type protein phosphatase gene ScPP2C information SEQ ID NO: 1, use biological software Primer5.0, Oligo 7 to design primers, the primers are composed of upstream primer GP-F and downstream primer GP-R, and the upstream primer GP The base sequence of -F is shown in SEQ ID NO: 2, the base sequence of the downstream primer GP-R is shown in SEQ ID NO: 3, and the primer was sent to Shanghai Sangong Company for synthesis.

[0027] 2. Total RNA was extracted from leaves of sugarcane thin-stem wild species treated with drought

[0028] (1) Processing of samples

[0029] Plant the wild sugarcane thin-stem seedlings in flowerpots, divide the planted sugarcane t...

Embodiment 2

[0064] Embodiment 2 A method for detecting the differential expression of the 2C-type protein phosphatase gene ScPP2C in the wild species of sugarcane slender-stemmed drought stress provided by the present invention comprises the following steps:

[0065] (1) Drought treatment:

[0066] 1. the sugarcane thin-stem wild species planted in flower pots (50cm in flowerpot diameter, height 30cm) is divided into experimental group and control group, 3 treatments of experimental components, wherein, processing 1: treat described sugarcane thin-stem wild species When the seedling grows to a height of 15-20 cm, the drought treatment is continuously carried out for 2 days; treatment 2: when the wild seedling of the sugarcane thin stem grows to a height of 15-20 cm, the drought treatment is continuously carried out for 4 days; When the wild seedlings grow to a height of 15-20 cm, the drought treatment is continuously carried out for 6 days; the drought treatment is: the three treatments a...

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Abstract

The invention discloses a saccharum spontaneum L. drought stress expressed protein phosphatase 2C gene ScPP2C. The base sequence of the protein phosphatase 2C gene ScPP2C is shown as SEQ ID NO:1. The gene directly takes part in the drought-resisting response process of the saccharum spontaneum L., and is a gene directly related to drought resistance. The saccharum spontaneum L. drought stress expressed protein phosphatase 2C gene ScPP2C establishes a base for deeply researching the drought-resisting mechanism of the gene in saccharum spontaneum L..

Description

technical field [0001] The invention belongs to the technical field of biomolecular cloning, and specifically relates to a gene encoding 2C-type protein phosphatase in sugarcane thin-stem wild species under drought stress, and primers are designed based on the sequence of the gene, and the 2C-type protein phosphatase is detected by semi-quantitative RT-PCR Method for differential expression of gene ScPP2C in sugarcane slender wild species. Background technique [0002] During the growth and development of sugarcane, it will encounter adversity stresses such as low temperature and drought, which seriously restrict the growth, sugar content and biomass of sugarcane. Therefore, how to improve the resistance of sugarcane to adversity stress and cultivate new varieties with strong stress resistance has become an important topic in the research of sugarcane genetics and breeding. Sugarcane thin-stem wild species (Saccharum spontaneum L.) is the most widely distributed species in ...

Claims

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Application Information

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IPC IPC(8): C12N15/55C12Q1/68
CPCC12N9/16C12Q1/686C12Q1/6895C12Q2521/107C12Q2545/114
Inventor 李富生徐荣何丽莲申明明程志远曹哲群
Owner YUNNAN AGRICULTURAL UNIVERSITY
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