10 results about "Saccharum edule" patented technology

The invention provides a primer group and a kit for detecting consanguinity of a saccharum arundinaceum chromosome 4 in sugarcane and application of the primer group and the kit, and belongs to the technical field of molecular biology. The primer group provided by the invention comprises 24 pairs of primers, and the sequences of the primers are shown as Seq1 to Seq48. The primer group, the kit and the application thereof provided by the invention can be used for rapidly detecting whether a sugarcane sample to be detected contains the specific interval consanguinity of the saccharum arundinaceum chromosome 4, have the characteristics of low cost and high sensitivity, and are of great significance in saccharum arundinaceum consanguinity detection in sugarcane filial generations, sugarcane breeding and the like.

This invention discloses a sugarcane salt tolerance gene. ShPP2C.F2-1 Its encoded proteins and applications, sugarcane salt tolerance genes ShPP2C.F2‑ 1 The nucleotide sequence of the gene is shown in SEQ ID NO:1, and the amino acid sequence of the protein it encodes is shown in SEQ ID NO:2. This gene enhances plant salt tolerance through a dual pathway of peroxidase-led antioxidant system and ion homeostasis regulation. Overexpression of this gene in rice significantly increased peroxidase activity, significantly decreased reactive oxygen species and malondialdehyde (MDA) content, while reducing sodium ion accumulation and stabilizing potassium ion levels, ultimately improving the survival rate of rice seedlings under salt stress. This invention clarifies... ShPP2C.F2-1 The salt tolerance function and core regulatory mechanism of genes provide new gene resources and key technical support for the genetic improvement of salt tolerance in crops such as sugarcane and rice, and have important application value for breeding new salt-tolerant crop varieties.

The invention provides a primer combination for identifying specific interval blood relationship of saccharum arundinaceum chromosome 10 in sugarcane, application and a method, and belongs to the technical field of molecular biology. The primer combination provided by the invention comprises 18 primer pairs, the sequences of the primer pairs are shown as Seq1-Seq36, and each primer pair consists of a forward primer and a reverse primer. According to the invention, every 30Mb of the saccharum arundinaceum chromosome 10 interval contains at least one pair of specific primers, so that the blood relationship of the specific saccharum arundinaceum chromosome 10 interval can be accurately identified, and the method has the advantages of high sensitivity, strong specificity, low cost, simplicity, convenience, rapidness and the like; technical support is provided for hybridization generation consanguinity identification of wild germplasm resource saccharum arundinaceum introduced from sugarcane in China, saccharum arundinaceum chromosome structure variation and other basic research, and the method has important significance on sugarcane breeding.

The invention provides a sugarcane ScGIP1 gene, an encoded protein and application, and belongs to the technical field of genetic engineering. The nucleotide sequence of the ScGIP1 gene is as shown in SEQ ID NO. 1. The invention preliminarily reveals that the sugarcane aspartic protease ScGIP1 as a novel plant disease-resistant factor has potential application value in improvement of plant disease resistance, enriches functional cognition of aspartic protease in plant immune response, provides potential gene resources for disease prevention and control of sugarcane and other crops, and has broad application prospects. Good application prospects and popularization values are realized.

The invention relates to the technical field of agricultural biology, in particular to an application of a BAG3 (Bcl-2-aspartic acid thiogene 3) gene in sugarcane (Saccharum spp.) or an encoding protein of the BAG3 gene in improvement of the resistance of plants to pathogens (especially viruses), and belongs to the technical field of agricultural biology and application of the Bcl-2-aspartic acid thiogene 3 gene and the encoding protein of the Bcl-2-aspartic acid thiogene 3 gene and the encoding protein of the Bcl-2-aspartic acid thiogene 3 gene. The invention provides an application of sugarcane BAG3 in plant virus resistance, and the sugarcane BAG3 provided by the invention has sugarcane streak mosaic virus resistance, and can activate plants to effectively resist plant virus infection.

To provide a method for producing a mixture of high-molecular weight compounds obtained from sugarcane wax and having neuroprotective action and antioxidant action.SOLUTION: Saponifying sugarcane wax with an alkaline or alkaline-earth hydroxide, subsequently subjecting the saponified wax obtained to extraction with CO2 in supercritical conditions, subjecting both the alcohol and aldehyde fraction extracted with CO2 and the mixture of residual salts to a purification step with hexane and acetone, analyzing the fractions obtained by gas chromatography and spectrophotometry in order to measure and determine the content of salts, alcohols and aldehydes, and then mixing in the proportions required to achieve the specific composition of the mixture.SELECTED DRAWING: None

Provided are a primer, a kit, and a method for determining whether a sugarcane material contains chromosome 7 of saccharum arundinaceum. The method uses a saccharum arundinaceum-specific sequence PCR primer TaChr07-F / R (the sequence is shown as SEQ ID NOs. 1-2) to stably test for chromosome 7 in a sugarcane material by means of a conventional, common PCR protocol. The method has the advantages of high efficiency, good stability, low costs, simple operation, and broad applicability, and can be applied to improving sugarcane germplasm resources or sugarcane breeding.

The present invention relates to the field of biotechnology. More precisely, a genetic construct and method for producing a transgenic plant event, especially a sugarcane event {Saccharum spp.), which is resistant to infestation by the Diatraea saccharalis pest, popularly known as a pest, ordinary borer, reed borer or just borer is described. The invention describes the event, the methods for event identification as well as the insertion detection method based on the unique region of intersection between the insert and the host genome and the flanking regions that characterize it.

A method for tapping a yield potential of Saccharum officinarum by controlling a time of plastic mulching is provided. The method includes: (1) plastic mulching: mulching a Saccharum officinarum furrow in a Saccharum officinarum field fully with a wide plastic film in a direction parallel to the Saccharum officinarum furrow, and covering the plastic film directly above the Saccharum officinarum furrow with soil with a certain thickness along the Saccharum officinarum furrow, such that the plastic film is tightly attached to soil in the Saccharum officinarum furrow; (2) control of the time of plastic mulching, and (3) plastic film recycling treatment. The method of the present disclosure is advanced, practical, easy to operate, and easy to promote.

The invention provides segmented PCR primers and a kit for detecting consanguinity of a saccharum arundinaceum chromosome 9 in sugarcane and application of the segmented PCR primers and the kit, and belongs to the technical field of molecular biology. The segmented PCR primer provided by the invention comprises 14 primer pairs, and the sequences of the primer pairs are shown as Seq1 to Seq28. According to the invention, at least one pair of specific primers is included in every 30Mb of the saccharum arundinaceum chromosome 9 interval and comprehensively covers the saccharum arundinaceum chromosome 9, and the provided segmented PCR primers can rapidly and comprehensively detect or identify whether the sugarcane and saccharum arundinaceum filial generation contains the specific interval consanguinity of the saccharum arundinaceum chromosome 9; technical support is provided for hybridization generation consanguinity identification of wild germplasm resource saccharum arundinaceum introduced from sugarcane in China and basic research on saccharum arundinaceum chromosome structure variation and the like, and the method has important significance on sugarcane breeding.