30 results about "Saccharum edule" patented technology

The invention discloses primers, a kit and a method for identifying whether a sugarcane material contains a saccharum arundinaceum chromosome 7 or not. According to the method, whether the sugarcane material contains the saccharum arundinaceum chromosome 7 or not can be stably detected by utilizing the saccharum arundinaceum specific sequence PCR primer TaChr07-F / R (the sequence is shown as SEQ ID NO.1-2) through a conventional common PCR reaction program, and the method has the advantages of high efficiency, good stability, low cost, simplicity and convenience in operation and good generalization performance, and can be applied to improvement of sugarcane germplasm resources or sugarcane breeding.

The invention discloses a breeding method of high-concentration cane molasses tolerant robust saccharomyces cerevisiae and high-concentration ethanol fermentation application of the high-concentration cane molasses tolerant robust saccharomyces cerevisiae, and belongs to the technical field of biology. The Saccharomyces cerevisiae NGTM-F1 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on July 25, 2023, and the number of the Saccharomyces cerevisiae NGTM-F1 is GDMCC 63687. The Saccharomyces cerevisiae NGTM-F1 disclosed by the invention is named as Saccharomyces cerevisiae NGTM-F1. The saccharomyces cerevisiae is obtained by taking laboratory saccharomyces cerevisiae GJ08 as an original strain, carrying out ARTP (room temperature plasma) mutagenesis and adaptive evolution breeding and utilizing high-throughput screening, keeps a good growth state in cane molasses with the total sugar being 300 g / L, and has good fermentation performance in cane molasses with the total sugar being 250 g / L at the temperature of 30 DEG C. Under the condition of 30 DEG C in a conical flask, the total sugar consumed by fermentation is 250 g / L, the ethanol concentration can reach 102.30 g / L, and the fermentation efficiency is 80.8%; under the condition of 30 DEG C in a 5 L fermentation tank, the total sugar consumed by fermentation is 250 g / L, the ethanol concentration can reach 111.65 g / L, and the fermentation efficiency is 87.0%.

The invention discloses a Burkholderia sp. Strain and an application of the Burkholderia sp. Strain. The Burkholderia sp. Strain is Burkholderia sp., the strain number of the Burkholderia sp. Strain is B58, and the registration number of the Burkholderia sp. Strain in the China General Microbiological Culture Collection Center is CGMCC No. 26956. The Burkholderia sp. B58 provided by the invention has a prevention and treatment effect on various pathogenic bacteria, and extracellular metabolites of the Burkholderia sp. B58 have a remarkable prevention and treatment effect on the sugarcane smut disease and have potential biocontrol application potential.

The invention belongs to the technical field of health-care food, and particularly relates to a sacha inchi oil-based gel candy with functions of reducing hypertension, hyperglycemia and hyperlipidemia and a preparation method thereof, and the sacha inchi oil-based gel candy comprises the following components: 1) a main component: 30-70% of sacha inchi oil; 2) a blood fat reducing synergistic component which is one or a combination of more of 0.5%-5% of phytosterol, 0.1%-2% of a red yeast rice extract and 0.01%-0.5% of sugarcane octacosanol; 3) a hypoglycemic synergistic component, which comprises one or a combination of more of 5%-20% of inulin / fructo-oligosaccharide, 0.5%-5% of a bitter gourd extract, 0.5%-5% of a mulberry leaf extract and 10-200 [mu] g / grain of chromium; 4) an antihypertensive synergistic component, wherein the antihypertensive synergistic component is one or a combination of more of 500-5000FU / grain of nattokinase, 0.5-5% of a celery seed extract and 1-8% of a hawthorn extract; through scientific compounding, the sacha inchi oil and various synergistic components exert a combined effect, and meanwhile, auxiliary regulation is carried out on blood sugar, blood fat and blood pressure; the main components are derived from natural plant extracts or functional grease, so that the health-care product is high in safety and suitable for daily health care.

A fuel pellet is disclosed. The fuel pellet comprises a plant fiber and a lignin modifying enzyme exogenous to the plant fiber. In some embodiments, the plant fiber is a Tracheophytes plant fiber. In some embodiments, the plant fiber is a monocot plant fiber. In some embodiments, the plant fiber is a Poaceae plant fiber. In some embodiments, the plant fiber is a Saccharum plant fiber. In some embodiments, the plant fiber is sugarcane bagasse plant fiber. In some embodiments, the pellet does not contain a binding agent. In embodiments without a binding agent, the fuel pellet is held together by lignin, which is present in the plant fiber and modified to hold the plant fibers together. In some embodiments, the lignin modifying enzyme is a lignase. In some other embodiments the lignin modifying enzyme is a laccase. The disclosure further provides a method of making a fuel pellet.

The invention discloses a roxburgh rose and sugarcane composite fruit wine fermentation method which comprises the following steps: mixing roxburgh rose normal juice and sugarcane normal juice according to a volume ratio of 1: 3-3: 1 to obtain mixed fruit juice, adjusting the pH value of the mixed fruit juice to 4.5-5.0 and the initial sugar degree to 22-26 degrees BX, inoculating activated yeast according to 0.3-0.5% of the mass of the mixed fruit juice, and fermenting at 22-26 DEG C for 8-10 days.

The invention provides a primer group and a kit for detecting consanguinity of a saccharum arundinaceum chromosome 4 in sugarcane and application of the primer group and the kit, and belongs to the technical field of molecular biology. The primer group provided by the invention comprises 24 pairs of primers, and the sequences of the primers are shown as Seq1 to Seq48. The primer group, the kit and the application thereof provided by the invention can be used for rapidly detecting whether a sugarcane sample to be detected contains the specific interval consanguinity of the saccharum arundinaceum chromosome 4, have the characteristics of low cost and high sensitivity, and are of great significance in saccharum arundinaceum consanguinity detection in sugarcane filial generations, sugarcane breeding and the like.

The invention provides refined molasses as well as a preparation method and application thereof, and relates to the technical field of molasses refining. The preparation method of the sugar honey comprises the following steps: S1, dissolving cane molasses in water, and then adding a strain for fermentation to obtain fermented molasses; the strains comprise bacillus subtilis and saccharomyces cerevisiae; s2, adjusting the pH value of the fermented molasses to 8-9, standing, centrifuging, removing lower-layer precipitate, and retaining supernatant; s3, adjusting the pH value of the supernatant to 5.5-7, and performing ultrafiltration and concentration to obtain a concentrated solution; and S4, sterilizing and spray-drying the concentrated solution to obtain the refined molasses. The refined molasses prepared by the method is high in reducing sugar and crude protein content and low in salt and ash content.

The invention discloses a PCR (Polymerase Chain Reaction) primer, a kit and a detection method for rapidly identifying whether sugarcane contains a saccharum arundinaceum chromosome 1 or not. The PCR primer is TaChr01-F / R, the nucleotide sequence of the PCR primer is shown as SEQ ID NO: 1-2, and through the PCR primer, whether a sugarcane material contains the saccharum arundinaceum chromosome 1 or not can be rapidly detected only by adopting a common PCR technology. The detection method disclosed by the invention is high in efficiency, good in stability, simple and convenient to operate, and very high in practicability and generalization performance.

The invention discloses a PCR (Polymerase Chain Reaction) primer, a kit and a detection method for rapidly identifying whether sugarcane contains a saccharum arundinaceum chromosome 5 or not. The PCR primer is TaChr05-F / R, the nucleotide sequence of the PCR primer is shown as SEQ ID NO: 1-2, and through the PCR primer, whether a sugarcane material contains the saccharum arundinaceum chromosome 5 or not can be rapidly detected only by adopting a common PCR technology. The detection method disclosed by the invention is high in efficiency, good in stability, simple and convenient to operate, and very high in practicability and generalization performance.

This invention discloses a sugarcane salt tolerance gene. ShPP2C.F2-1 Its encoded proteins and applications, sugarcane salt tolerance genes ShPP2C.F2‑ 1 The nucleotide sequence of the gene is shown in SEQ ID NO:1, and the amino acid sequence of the protein it encodes is shown in SEQ ID NO:2. This gene enhances plant salt tolerance through a dual pathway of peroxidase-led antioxidant system and ion homeostasis regulation. Overexpression of this gene in rice significantly increased peroxidase activity, significantly decreased reactive oxygen species and malondialdehyde (MDA) content, while reducing sodium ion accumulation and stabilizing potassium ion levels, ultimately improving the survival rate of rice seedlings under salt stress. This invention clarifies... ShPP2C.F2-1 The salt tolerance function and core regulatory mechanism of genes provide new gene resources and key technical support for the genetic improvement of salt tolerance in crops such as sugarcane and rice, and have important application value for breeding new salt-tolerant crop varieties.

The invention provides a primer combination for identifying specific interval blood relationship of saccharum arundinaceum chromosome 10 in sugarcane, application and a method, and belongs to the technical field of molecular biology. The primer combination provided by the invention comprises 18 primer pairs, the sequences of the primer pairs are shown as Seq1-Seq36, and each primer pair consists of a forward primer and a reverse primer. According to the invention, every 30Mb of the saccharum arundinaceum chromosome 10 interval contains at least one pair of specific primers, so that the blood relationship of the specific saccharum arundinaceum chromosome 10 interval can be accurately identified, and the method has the advantages of high sensitivity, strong specificity, low cost, simplicity, convenience, rapidness and the like; technical support is provided for hybridization generation consanguinity identification of wild germplasm resource saccharum arundinaceum introduced from sugarcane in China, saccharum arundinaceum chromosome structure variation and other basic research, and the method has important significance on sugarcane breeding.

Compositions and methods for increasing fatty acid and / or oil accumulation in the stems of plants are provided. Constructs including stem cell-specific promoters operably linked to nucleic acids encoding a plastidic phosphoenolpyruvate / phosphate translocator protein and a tonoplast-localized hexose transporter protein are described.

The invention provides a sugarcane ScGIP1 gene, an encoded protein and application, and belongs to the technical field of genetic engineering. The nucleotide sequence of the ScGIP1 gene is as shown in SEQ ID NO. 1. The invention preliminarily reveals that the sugarcane aspartic protease ScGIP1 as a novel plant disease-resistant factor has potential application value in improvement of plant disease resistance, enriches functional cognition of aspartic protease in plant immune response, provides potential gene resources for disease prevention and control of sugarcane and other crops, and has broad application prospects. Good application prospects and popularization values are realized.

The invention discloses a PCR (Polymerase Chain Reaction) primer, a kit and a detection method for rapidly identifying whether sugarcane contains a saccharum arundinaceum chromosome 2 or not. The PCR primer is TaChr02-F / R, the nucleotide sequence of the PCR primer is shown as SEQ ID NO: 1-2, and through the PCR primer, whether a sugarcane material contains the saccharum arundinaceum chromosome 2 or not can be rapidly detected only by adopting a common PCR technology. The detection method disclosed by the invention is high in efficiency, good in stability, simple and convenient to operate, and very high in practicability and generalization performance.

The invention relates to the technical field of agricultural biology, in particular to an application of a BAG3 (Bcl-2-aspartic acid thiogene 3) gene in sugarcane (Saccharum spp.) or an encoding protein of the BAG3 gene in improvement of the resistance of plants to pathogens (especially viruses), and belongs to the technical field of agricultural biology and application of the Bcl-2-aspartic acid thiogene 3 gene and the encoding protein of the Bcl-2-aspartic acid thiogene 3 gene and the encoding protein of the Bcl-2-aspartic acid thiogene 3 gene. The invention provides an application of sugarcane BAG3 in plant virus resistance, and the sugarcane BAG3 provided by the invention has sugarcane streak mosaic virus resistance, and can activate plants to effectively resist plant virus infection.

The present invention provides refined molasses, a preparation method, and applications thereof, relating to the technical field of molasses refining. The molasses preparation method of the present invention comprises the following steps: S1. dissolving sugarcane molasses in water, adding bacterial strains for fermentation, and obtaining fermented molasses; the bacterial strains include Bacillus subtilis and Saccharomyces cerevisiae; S2. adjusting the pH of the fermented molasses to 8-9, allowing it to stand, and then centrifuging, discarding the lower precipitate and retaining the upper liquid; S3. adjusting the pH of the upper liquid to 5.5-7, ultrafiltration, and concentration to obtain a concentrated solution; S4. sterilizing and spray-drying the concentrated solution to obtain refined molasses. The refined molasses prepared by this method has high reducing sugar and crude protein contents and low salt and ash contents.

The invention provides a traditional Chinese medicine composition for treating chronic pharyngitis, a traditional Chinese medicine preparation and a preparation method thereof, and the traditional Chinese medicine composition comprises the following components in parts by weight: 2-80 parts of physalis alkekengi, 1-70 parts of sugarcane, 1-60 parts of laver, 1-50 parts of lily, 1-50 parts of American ginseng, 1-50 parts of pseudo-ginseng, 1-40 parts of pericarpium citri reticulatae, 1-40 parts of semen coicis and 1-40 parts of lotus seed. The invention further provides a traditional Chinese medicine preparation for treating chronic pharyngitis and a preparation method thereof. The traditional Chinese medicine composition has a remarkable curative effect on chronic pharyngitis, the raw materials of the traditional Chinese medicine composition are all medicinal and edible varieties, are high in safety, free of toxicity and side effects, wide in raw material source, easy to obtain and suitable for being taken for a long time, and the preparation method of the traditional Chinese medicine composition is easy and convenient to operate, can achieve large-scale industrial production and is beneficial to industrial application of the composition.

The invention provides a primer combination for identifying consanguinity of saccharum arundinaceum chromosome 2 and application of the primer combination, and belongs to the technical field of plant consanguinity identification. The primer combination provided by the invention comprises 14 primer pairs, and every 30Mb of specific primers in a chromosome 2 interval of saccharum arundinaceum at least comprises one primer pair. The method overcomes the defects of a traditional sugarcane and saccharum arundinaceum filial generation consanguinity detection and identification method, can accurately identify whether a sample to be detected contains a specific consanguinity interval of the saccharum arundinaceum chromosome 2, has the advantages of high sensitivity, strong specificity, low cost, simplicity, convenience, rapidness and the like, and can be widely applied to detection and identification of the consanguinity of the saccharum arundinaceum filial generation. The method can be used for blood relationship identification and high-throughput rapid detection of a specific interval of the saccharum arundinaceum chromosome 2 in a sugarcane and saccharum arundinaceum filial generation.

Disclosed herein is an advanced synergistic nano herbal composition having significant immunomodulatory and antiviral effects in human subjects, said formulation comprising the specific combination of HAVEs of Citrus limon, Cymbopogon citratus, Origanum vulgare, Thymus serpyllum, Tinospora cordifolia, Withania somnifera, Zea mays, Meyna laxiflora, Blumea lacera, Boswellia serrata, Cinnamomum verum, Mentha pipperata, Mentha arvensis, Nigella sativa, Cocos nucifera, Fagonia arabica, Azadirachta indica, Eucalyptus globulus, Citrus sinensis, Coriandrum sativum, Saccharum officinarum, Syzygium aromaticum, Dolichos biflorus. Resulting composition is sonicated to obtain the final advanced synergistic nano herbal composition having average particle size 133.2nm.

The invention discloses a bacterial strain, which is Saccharomyces cerevisiae GH-Y16, is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is January 22, 2024, and the preservation number is CGMCC No.29719. The invention further discloses a preparation method of the bacterial strain. The invention relates to a method for preparing sugarcane polyphenol through fermentation, which comprises the following steps: preparing saccharomyces cerevisiae into a seed solution, inoculating the seed solution into sugarcane juice or molasses diluent for fermentation, and after the fermentation is finished, filtering the fermentation liquor to remove impurities, and concentrating in vacuum to obtain the sugarcane polyphenol. The saccharomyces cerevisiae is saccharomyces cerevisiae GH-Y16. The method can replace the traditional chemical extraction method combined with the chromatographic purification method to prepare the sugarcane polyphenol, and has the advantages of reducing the production cost of the product, reducing the danger coefficient of the production environment, avoiding the chemical residue of the product, reducing the environmental pollution and the like.

To provide a method for producing a mixture of high-molecular weight compounds obtained from sugarcane wax and having neuroprotective action and antioxidant action.SOLUTION: Saponifying sugarcane wax with an alkaline or alkaline-earth hydroxide, subsequently subjecting the saponified wax obtained to extraction with CO2 in supercritical conditions, subjecting both the alcohol and aldehyde fraction extracted with CO2 and the mixture of residual salts to a purification step with hexane and acetone, analyzing the fractions obtained by gas chromatography and spectrophotometry in order to measure and determine the content of salts, alcohols and aldehydes, and then mixing in the proportions required to achieve the specific composition of the mixture.SELECTED DRAWING: None

The invention discloses a PCR (Polymerase Chain Reaction) primer, a kit and a detection method for rapidly identifying whether sugarcane contains a saccharum arundinaceum chromosome 4 or not. The PCR primer is TaChr04-F / R, the nucleotide sequence of the PCR primer is shown as SEQ ID NO: 1-2, and through the PCR primer, whether a sugarcane material contains the saccharum arundinaceum chromosome 4 or not can be rapidly detected only by adopting a common PCR technology. The detection method disclosed by the invention is high in efficiency, good in stability, simple and convenient to operate, and very high in practicability and generalization performance.

Provided are a primer, a kit, and a method for determining whether a sugarcane material contains chromosome 7 of saccharum arundinaceum. The method uses a saccharum arundinaceum-specific sequence PCR primer TaChr07-F / R (the sequence is shown as SEQ ID NOs. 1-2) to stably test for chromosome 7 in a sugarcane material by means of a conventional, common PCR protocol. The method has the advantages of high efficiency, good stability, low costs, simple operation, and broad applicability, and can be applied to improving sugarcane germplasm resources or sugarcane breeding.

The present invention relates to the field of biotechnology. More precisely, a genetic construct and method for producing a transgenic plant event, especially a sugarcane event {Saccharum spp.), which is resistant to infestation by the Diatraea saccharalis pest, popularly known as a pest, ordinary borer, reed borer or just borer is described. The invention describes the event, the methods for event identification as well as the insertion detection method based on the unique region of intersection between the insert and the host genome and the flanking regions that characterize it.

The invention discloses a primer, a kit and a method for identifying whether a sugarcane material contains a saccharum arundinaceum chromosome 10 or not. According to the method, the saccharum arundinaceum specific sequence PCR primer TaChr10-F / R (the sequence is shown as SEQ ID NO.1-2) is utilized to stably detect whether the sugarcane material contains the saccharum arundinaceum chromosome 10 or not through a conventional common PCR reaction program, and the method has the advantages of being high in efficiency, good in stability, low in cost, easy and convenient to operate and good in generalization performance and can be applied to sugarcane germplasm resource improvement or sugarcane breeding.

The invention discloses a primer, a kit and a method for identifying whether a sugarcane material contains a saccharum arundinaceum chromosome 6 or not. According to the method, whether the sugarcane material contains the saccharum arundinaceum chromosome 6 or not can be stably detected by utilizing the saccharum arundinaceum specific sequence PCR primer TaChr06-F / R (the sequence is shown as SEQ ID NO.1-2) through a conventional common PCR reaction program, and the method has the advantages of high efficiency, good stability, low cost, simplicity and convenience in operation and good generalization performance, and can be applied to improvement of sugarcane germplasm resources or sugarcane breeding.

A method for tapping a yield potential of Saccharum officinarum by controlling a time of plastic mulching is provided. The method includes: (1) plastic mulching: mulching a Saccharum officinarum furrow in a Saccharum officinarum field fully with a wide plastic film in a direction parallel to the Saccharum officinarum furrow, and covering the plastic film directly above the Saccharum officinarum furrow with soil with a certain thickness along the Saccharum officinarum furrow, such that the plastic film is tightly attached to soil in the Saccharum officinarum furrow; (2) control of the time of plastic mulching, and (3) plastic film recycling treatment. The method of the present disclosure is advanced, practical, easy to operate, and easy to promote.

A smoking product comprises a substrate, at least one flavouring, a mist-maker, and an absorbent plant-based material dispersed within the smoking product. The absorbent plant-based material is Saccha

The invention provides segmented PCR primers and a kit for detecting consanguinity of a saccharum arundinaceum chromosome 9 in sugarcane and application of the segmented PCR primers and the kit, and belongs to the technical field of molecular biology. The segmented PCR primer provided by the invention comprises 14 primer pairs, and the sequences of the primer pairs are shown as Seq1 to Seq28. According to the invention, at least one pair of specific primers is included in every 30Mb of the saccharum arundinaceum chromosome 9 interval and comprehensively covers the saccharum arundinaceum chromosome 9, and the provided segmented PCR primers can rapidly and comprehensively detect or identify whether the sugarcane and saccharum arundinaceum filial generation contains the specific interval consanguinity of the saccharum arundinaceum chromosome 9; technical support is provided for hybridization generation consanguinity identification of wild germplasm resource saccharum arundinaceum introduced from sugarcane in China and basic research on saccharum arundinaceum chromosome structure variation and the like, and the method has important significance on sugarcane breeding.