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Method for distinguishing transgenic potatoes

A potato, transgenic technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. handy effect

Inactive Publication Date: 2017-12-15
山东世通检测评价技术服务有限公司
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Problems solved by technology

However, in the detection process of genetically modified products, multiple exogenous genes are often screened and detected. Although the ordinary PCR method has obvious advantages in the detection of genetically modified products, it is time-consuming, cumbersome and expensive for the detection of multiple exogenous genes. major issues
[0004] At present, the detection of transgenic potato is limited to the combination of a single exogenous gene or a small number of regulatory elements and target genes, and there is no multiplex PCR technology for detecting the combination of regulatory elements in transgenic potato

Method used

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  • Method for distinguishing transgenic potatoes
  • Method for distinguishing transgenic potatoes
  • Method for distinguishing transgenic potatoes

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Embodiment 1

[0037] Example 1: Primer Design and Screening:

[0038] By searching relevant literature and standards, 8 pairs of regulatory elements commonly used in transgenic crops were searched, relevant sequence information was searched through NCBI, and its conserved regions were searched. According to the sequences of the conserved regions found, primer sequences were designed using Primer5. the primer sequence. The 8 pairs of primers designed were screened by PCR, and finally 4 kinds of regulatory elements commonly used in transgenic potato were screened out. The sequence information of the primers is shown in Table 1.

[0039] Table 1 Primer sequence information

[0040]

[0041]

[0042] The designed primers were verified for single gene (A, B, C, D) and two pairs of primer combinations (AB, AC, BC, BD, CD). Among them, the final concentration of each primer was 0.4 μmol / L for single-gene verification, and 0.3 μmol / L for two-pair primer combination verification.

[0043] 2...

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Abstract

The invention belongs to the technical field of transgenic product detection, and particularly relates to a PCR detection method for commonly-used regulatory elements in transgenic potatoes. The specificity of the primers of the method is strong, and through the mode of combining two pairs of composite primers and single primers, PCR detection is conducted on a potato sample; agarose gel electrophoresis is used for analyzing a PCR product, the stripe size of the PCR product can be obviously distinguished, and the synchronous detection of the multiple regulatory elements of the transgenic potatoes is achieved. The detection method has the advantages of simple operation, high sensitivity, low costs and the like, and a fast, effective and reliable detection method is provided for transgenic agricultural products. The screened primer combination and the detection method not only are suitable for potato sample detection, but also can be expanded to the detection of other transgenic agricultural products.

Description

technical field [0001] The invention belongs to the technical field of transgenic product detection, and in particular relates to a method for identifying transgenic potatoes. Background technique [0002] Potato is the fourth largest food crop after rice, wheat and corn in the world. At present, there are more than 40 kinds of genetically modified potatoes approved for commercialization in the world, mainly involving traits such as variety improvement, insect resistance and disease resistance. With the gradual commercialization of genetically modified crops, while bringing huge economic benefits to people, food safety issues are also worrying, which brings great challenges and market demand to the detection of genetically modified products. How to quickly, accurately and sensitively identify genetically modified samples is a major problem currently facing the genetically modified detection industry. [0003] At present, the detection technologies of genetically modified p...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/16C12Q2537/143
Inventor 孙海新周延培孙丕春张慧崔玲君
Owner 山东世通检测评价技术服务有限公司
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