Method for improving quality of carbon dioxide expanded tobacco shreds by using perfumed compound microbiological agent
A technology of compounding microorganisms and carbon dioxide, which is applied in the fields of tobacco, application, and tobacco treatment, can solve the problems of high loss of aroma substances, affecting equipment performance, and improving the sensory quality of shredded tobacco, and achieves the goal of reducing the hazard index, reducing processing costs, and reducing the impact Effect
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Embodiment 1
[0023] Bacillus subtilis ( Bacillus subtilis ) Y1, Bacillus subtilis ( Bacillus subtilis ) X7 and Bacillus subtilis ( Bacillus subtilis ) Separation and screening of X8:
[0024] 1. Isolate the strains in the flavoring compound microbial preparation from the surface of natural alcoholized tobacco leaves, namely Bacillus subtilis ( Bacillus subtilis ) Y1, Bacillus subtilis ( Bacillus subtilis ) X7 and Bacillus subtilis ( Bacillus subtilis )X8, its separation method is: after first mixing 90mL sterile water with 10g alcoholized tobacco leaves (i.e. 10 -1 Suspension), use aseptic sampling method to take 1mL of the mixture and add it to 9mL sterile water to make 10 -2 suspension, and then serially diluted, take 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 Spread 0.2mL of suspensions of six dilutions on the improved LB medium, incubate at a constant temperature of 37°C for 24 hours, pick a single colony on a plate with good growth, full and uniform colonies, and appropriate ...
Embodiment 2
[0035] The bacillus subtilis that embodiment 1 obtains ( Bacillus subtilis ) Y1, Bacillus subtilis ( Bacillus subtilis ) X7, Bacillus subtilis ( Bacillus subtilis )X8 is compounded according to the mass ratio of 1:1:1 to obtain a fragrance-imparting microbial preparation, which is called preparation 1;
[0036] The bacillus subtilis that embodiment 1 obtains ( Bacillus subtilis ) Y1, Bacillus subtilis ( Bacillus subtilis ) X7, Bacillus subtilis ( Bacillus subtilis )X8 is compounded according to the mass ratio of 0.001:1:0.001 to obtain a fragrance-imparting microbial preparation, which is called preparation 2;
[0037] The bacillus subtilis that embodiment 1 obtains ( Bacillus subtilis ) Y1, Bacillus subtilis ( Bacillus subtilis ) X7, Bacillus subtilis ( Bacillus subtilis )X8 is compounded according to the mass ratio of 1:0.001:1 to obtain a fragrance-imparting microbial preparation, which is called preparation 3;
[0038] The bacillus subtilis that embodiment 1...
Embodiment 3
[0042] The composite microbial preparation prepared in Example 2 is used for the fermentation of carbon dioxide-expanded shredded tobacco, and the tobacco leaves / leaf groups are sliced, loosened and rehydrated, then humidified and shredded to obtain shredded tobacco, at 0.0001wt% to 0.5wt% of the shredded tobacco mass Weigh the composite microbial preparation prepared in Example 2, spray it on the shredded tobacco, and ferment it at a constant temperature and humidity for 24h to 60h at a temperature of 25°C to 45°C and a relative humidity of 30% to 90%. Shredded tobacco expansion treatment, carbon dioxide shredded tobacco expansion treatment is specifically: after feeding, the shredded tobacco is dipped for 100-200s, opened, and then at a temperature of 240°C-320°C, a steam flow rate of 550 KG / H-650KG / H, and a process wind speed of 30m / s The shredded tobacco is expanded under the condition of ~40m / s, and then the shredded tobacco is obtained after cooling and dampening the shre...
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