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A kind of method for preparing peptone using fish meal pressing liquid

A technology for preparing peptone and pressing liquid, which is applied in the field of preparing peptone by using fish meal pressing liquid, can solve problems such as affecting breeding, perishability, ecological environment pollution, etc., and achieves the effects of strong operability, efficient production and advanced technology

Active Publication Date: 2021-01-22
荣成市日鑫水产有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because fishmeal wastewater contains solids such as dissolved fish protein, fish oil, amino acids, trace elements and other components that are easily corrupted, it has caused the eutrophication of my country's coastal waters, caused serious pollution to the ecological environment, and affected my country's coastal beaches and offshore aquaculture.

Method used

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  • A kind of method for preparing peptone using fish meal pressing liquid
  • A kind of method for preparing peptone using fish meal pressing liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of method that utilizes fishmeal squeeze liquid to prepare peptone, it goes through the following steps:

[0024] (1) Select fresh, pollution-free, normal-smelling fishmeal squeezed liquid, put it into a static tank, and remove fish oil by standing;

[0025] (II) Concentrate the fishmeal squeezed liquid obtained in step I under negative pressure and at 5-10 degrees Celsius until the solid content is 16-18% by wet weight, and the weight of the concentrated mixture is 10,000 parts by weight (here due to the concentration equipment Due to the limit, it is impossible to concentrate all at one time, so the weight of solids in each concentration result is different. And because it is impossible to perform a complete solids separation test, the measurement result is a sampling measurement, which has a large uncertainty , only one range of values ​​can be obtained. Similarly, the concentration temperature is controlled within the range of 5-10 degrees Celsius, and it is ...

Embodiment 2

[0032] A kind of method that utilizes fishmeal squeeze liquid to prepare peptone, it goes through the following steps:

[0033] (1) Select fresh, pollution-free, normal-smelling fishmeal squeezed liquid, put it into a static tank, and remove fish oil by standing;

[0034] (II) concentrating the squeezed liquid of fish meal obtained in step I under negative pressure and at 5-10 degrees Celsius until the solid content is 16-18% by wet weight, and the weight of the concentrated mixture is 10,000 parts by weight;

[0035] (III) Heating the concentrated solution obtained in step II to 40 degrees Celsius, and adding 45 parts by weight of citric acid to the concentrated solution;

[0036] (IV) Add acid protease to the mixture obtained in step III to 4500 U / mL, and enzymatically digest at 40 degrees Celsius for 4 hours;

[0037] (V) Add 60 parts by weight of disodium hydrogen phosphate and 600 parts by weight of bean dregs to the mixture obtained in step IV, add trypsin to 5000 U / mL,...

Embodiment 3

[0041] A kind of method that utilizes fishmeal squeeze liquid to prepare peptone, it goes through the following steps:

[0042] (1) Select fresh, pollution-free, normal-smelling fishmeal squeezed liquid, put it into a static tank, and remove fish oil by standing;

[0043] (II) concentrating the squeezed liquid of fish meal obtained in step I under negative pressure and at 5-10 degrees Celsius until the solid content is 16-18% by wet weight, and the weight of the concentrated mixture is 10,000 parts by weight;

[0044] (III) Heating the concentrated solution obtained in step II to 45 degrees Celsius, and adding 70 parts by weight of citric acid to the concentrated solution;

[0045] (IV) Add acid protease to the mixture obtained in step III to 2000 U / mL, and enzymatically digest at 45 degrees Celsius for 2 hours;

[0046] (V) Add 180 parts by weight of disodium hydrogen phosphate and 1200 parts by weight of bean dregs to the mixture obtained in step IV, add trypsin to 3000 U / m...

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PUM

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Abstract

The invention discloses a method for preparing peptone with squeezed fishmeal liquid. The method includes the following steps that (I) the squeezed fishmeal liquid is standing, and fish oil is removed; (II) the squeezed fishmeal liquid obtained in the step I is subjected to negative-pressure lower-temperature concentration; (III) the concentrated liquid obtained in the step II is heated, and citric acid is added into the concentrated liquid; (IV) acid protease is added into the mixture obtained in the step III, and enzymolysis is carried out; (V) sodium hydrogen phosphate and bean dregs are added into the mixture obtained in the step IV, trypsin is added, enzymolysis is carried out, and then inactivation is carried out; (VI) the fermentation mixture obtained in the step V is filtered through a plate-and-frame filter press; (VII) filtered liquid is concentrated, spraying and drying are carried out, and a peptone powder finished product is obtained. According to the method, as mixed enzymolysis is carried out by adding the bean dregs, and the enzymolysis condition is optimized, the peptone prepared with the method has the particularly-excellent bacterial culture medium characteristic. The method is reasonable, the technology is advanced, the operability is high, and the method can be widely used for manufacturing culture media of bacteria.

Description

technical field [0001] The invention relates to the preparation of peptides, in particular to a method for preparing peptone from fish powder squeeze liquid. Background technique [0002] We know that fishmeal is a high-protein feed raw material obtained by using one or more low-value fish as raw materials, pressing, deoiling, dehydrating, drying, and crushing after cooking. After being pressed, fishmeal waste water is discharged directly without treatment. Because fishmeal wastewater contains solids such as dissolved fish protein, fish oil, amino acids, trace elements and other components that are easily corrupted, it has caused the eutrophication of my country's offshore seawater, caused serious pollution to the ecological environment, and affected my country's coastal beaches and offshore aquaculture. . [0003] Therefore, how to use fish meal processing waste liquid to prepare peptone is an urgent problem to be solved. Contents of the invention [0004] In order to o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 房文涛原永广
Owner 荣成市日鑫水产有限公司