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Application of blue light in affecting the proliferation and differentiation of neural stem cells

A technology for neural stem cells and cell proliferation, applied in the directions of nervous system cells, animal cells, vertebrate cells, etc., can solve problems such as stem degeneration, and achieve the effect of reducing the degree of differentiation, low cytotoxic side effects, and broad application prospects.

Active Publication Date: 2020-07-03
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This application can solve the problem of stem degeneration of neural stem cells in vitro

Method used

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  • Application of blue light in affecting the proliferation and differentiation of neural stem cells
  • Application of blue light in affecting the proliferation and differentiation of neural stem cells
  • Application of blue light in affecting the proliferation and differentiation of neural stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] 1. Isolation and culture of fetal mouse whole brain neural stem cells

[0048] 1. Take a C57BL / 6 mouse at 13.5 days pregnant, kill it by neck dislocation, disinfect the whole body with 75% alcohol by volume, and then transfer it to a super-clean bench.

[0049] 2. The embryos were taken out by laparotomy, washed in pre-cooled PBS, and then operated on ice.

[0050] 3. Peel off the embryonic skull and take out the whole brain. After washing with PBS, shred it sufficiently to obtain the cell slurry.

[0051] 4. Filter the cell slurry with a 200-mesh sieve, filter it into a 10cm culture dish containing 10ml of neural stem cell proliferation medium (Table 1), mix well, and store at 37°C, 5% CO 2 Cultivated in a constant temperature and humidity incubator.

[0052] 5. Add 2ml neural stem cell proliferation medium to the culture dish every day to maintain the nutrients needed for cell growth.

[0053] Table 1: Components of Proliferation Medium

[0054]

[0055] Second...

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Abstract

The invention relates to the application of blue light in the proliferation and differentiation of neural stem cells. According to the application, after high-purity mouse neural stem cells obtainedthrough culture is irradiated with blue light under specific conditions, normal culture is conducted, it is found that the proliferation rate of the neural stem cells is increased significantly, and the number of cells differentiated into neurons are decreased significantly. Only through blue light irradiation under the specific conditions, the purposes of increasing the proliferation rate of theneural stem cells and reducing the differentiation degree of the neural stem cells are achieved for the first time, no other substances are added, no invasion and no contact exist, and the toxic and side effect on the cells is small; no invasion or modification operation is conducted on the neural stem cells, only physical conditions are made use of, and the blue light has a broad application prospect.

Description

technical field [0001] The invention relates to the application of blue light in influencing the proliferation and differentiation of neural stem cells, and belongs to the technical field of neural stem cell culture. Background technique [0002] Neural stem cells (NSCs) refer to the ability to differentiate into neurons, astrocytes, and oligodendrocytes, can self-renew, produce other cells other than themselves through asymmetric division, and are sufficient to Stem cells that provide a large number of brain tissue cells. Neural stem cells have position-specific differentiation potential, and their proliferation, differentiation and migration are closely related to extracellular matrix (ECM). The main biological characteristics of neural stem cells are: (1) multi-directional differentiation potential and in a highly undifferentiated state: they can differentiate into three main cells of the nervous system, namely neurons, astrocytes and oligodendrocytes. (2) Self-renewal,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0797
Inventor 李曦许志亮王梅天
Owner SHANDONG UNIV
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