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Aconitum coreanum DNA barcode based on big data and method for identifying aconitum coreanum

A technology of Aconitum and bar code, which is applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial determination/inspection, etc. The results have high accuracy, which is conducive to rapid identification and rapid detection process.

Active Publication Date: 2018-01-05
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Description
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Problems solved by technology

[0004] At present, in the research on the molecular identification of medicinal plants of the genus Aconitum, there is insufficient sample sampling during the construction of the species identification library, and the problem of similarities and differences of the variation sites between different individuals or among different individuals within the same species cannot be considered, which leads to the failure of identification. Poor reliability and low accuracy

Method used

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  • Aconitum coreanum DNA barcode based on big data and method for identifying aconitum coreanum
  • Aconitum coreanum DNA barcode based on big data and method for identifying aconitum coreanum
  • Aconitum coreanum DNA barcode based on big data and method for identifying aconitum coreanum

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Embodiment 1

[0041] 1. Collection and preservation of specimens of aconitum medicinal plants

[0042] Check the literature and the collection information of major domestic herbariums, and formulate a field sampling strategy according to the sampling requirements of DNA barcodes. Each species collects as many individuals (1-13) from different sources in its distribution area as possible. The leaves are dried and stored with silica gel. A total of 206 leaf materials of more than 70 species (including varieties) of Aconitum were obtained, and the sequence information of the remaining samples was downloaded from the NCBI gene bank (23 entries in total). Among them, the samples of Aconitum chinense came from 5 places in Heilongjiang Province, Jilin Province and Liaoning Province, covering the main distribution areas of the species, and 3 leaf samples were collected at each sample point. The list of materials and sample sources are shown in Table 1.

[0043] Table 1 Specimens and sources of medicin...

Embodiment 2

[0079] With the leaves of Aconitum vulgaris and other Aconitum plants collected in the medicinal material planting area and the field, the method of Example 1 was used for DNA extraction, PCR amplification and sequencing. The identification results showed that The 89 bp of the ITS sequence obtained from the Aconitum aconitum plant is base A, 443 bp is A, 447 bp is A, 491 bp and 492 bp are AT, 499 bp is T, 577 bp is C, and other unknown Aconitum plants do not see the above The specific sites can be used to identify Aconitum aconitum samples.

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Abstract

The invention discloses an aconitum coreanum DNA barcode based on big data and a method for identifying aconitum coreanum, and belongs to the technical field of molecular identification. Through extensively investigating a distribution area of aconitum medicinal plants and sampling in many places as far as possible in a species distribution area, a barcode big data base of ITS sequences of the aconitum medicinal plants is constructed, then a unique ITS sequence specific information site of aconitum coreanum is found through comparison, and further a unique DNA barcode of the aconitum coreanumis obtained; the DNA barcode is a sequence with a total length of 629 bp and obtained by amplification of primer sequences SEQ ID NO. 1 and SEQ ID NO. 2, the 89th bp is A, the 443rd bp is A, the 447thbp is A, the 491st bp and the 492nd bp are AT, the 499th bp is T, and the 577th bp is C. The DNA barcode can greatly facilitate the rapid identification of the medicinal plant aconitum coreanum.

Description

Technical field [0001] The present invention relates to the technical field of molecular identification, in particular to a large data-based aconitum aconitum DNA barcode, and a method for identifying aconitum aconitum by using the DNA barcode. Background technique [0002] Aconitum coreanum (H.Léveillé) Rapaics, also known as Guanfuzi, Baifuzi, mountain trumpet flower and yellow ura flower, is a perennial herbaceous medicinal plant belonging to the genus Aconitum in the Ranunculaceae family, especially in Changbai Mountain Authentic herbs. Its roots are used as medicine, which can expel wind, dry dampness, resolve phlegm and relieve pain. There are more than 200 species of Aconitum in our country. At the same time, all plants of this genus belong to poisonous plants. The phenomenon of the same name or the same name in the use of Aconitum medicinal materials is widespread. Domestic reports of Aconitum poisoning are common, and various Aconitum plants are difficult to identify b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12Q1/68
Inventor 杨俊波何俊王家艳王红李德铢
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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