Multi-channel fluorescence detecting system and method of using the same

Abstract

The present invention provides a multi-channel fluorescence detecting system for detecting a plurality of fluorescence labeled analytes. The multi-channel fluorescence detecting system comprises a light source, a light filter device, a dual branch light guide tube, and a detector. The light source comprises a plurality of sub light sources for respectively providing an excitation light. The plurality of sub light sources are a plurality of single color Light emitting diodes (LEDs) which can be selectively turned on or off. The light source generates a plurality of lights with full width at half maximum (FWHM) wavelengths formed in a non-overlap manner. With the disposition of the plurality of sub light sources, the accuracy for detecting the specific analytes is raised, the light flux witha specific wavelength band is effectively raised (without raising the light flux of the full wavelength band), the structure is simplified, and the manufacturing cost is decreased.

Description

technical field [0001] The invention relates to a multi-channel fluorescence detection system and its method, especially used in the field of biochemistry to detect specimens reacting to specific wavelength bands. Background technique [0002] In the field of biochemistry, it is common to use fluorescence detection technology to detect samples. Since the fluorescence detection method does not need to be in contact with the detection sample, it can avoid the operator from being exposed to a highly infectious environment, and perform non-destructive quantitative or qualitative measurements on the detection sample, so that the technology is widely used. [0003] Fluorescence detection uses certain components of the analyte to bond with a specific fluorescent dye, and the fluorescent dye has the characteristic of photoactivating light (Photoluminescence) for a specific wavelength in the spectrum, so the analysis sample is irradiated Under the incident light of fixed intensity, ...

AI Technical Summary

Problems solved by technology

[0009] Although the prior art can detect multiple wavelength bands simultaneously, there are several problems: 1. when the light is white light, redundant emission light will be produced; 2. because the emission filter 15 passes through, The emitted light may be filtered by wavelengths that do not need to be filtered, thereby affecting the accuracy of subsequent detection; 3. The emitted light filter 15 is made on the same piece of glass in a special way by using a multi-layer filter, Its manufacturing process and production cost are relatively high; 4. Although white light can be used to generate emitted light in multiple wavelength bands at the same time, it may also cause the emitted light 18 to generate light of unnecessary wavelengths, resulting in misjudgment

However, the design of synchronous detection not only increases the cost of the optical sensor and optical path, but also makes the overall sensing area bulky and complicated

[0018] As mentioned above, several LEDs have been used to synthesize white light in the prior art. However, because the fluorescence detection system of the prior art detects all the fluorescent labels at the same time, the several LEDs need to be turned on at the same time, otherwise it will not be possible. To achieve the purpose of synchronous detection

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