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RNA aptamer-based theophylline quantitative detection method

A quantitative detection method and RNA probe technology, applied in the field of theophylline detection, can solve the problems of low cost and use of large-scale equipment reagents, etc., and achieve the effects of high specificity, low interference electrochemical detection, and high sensitivity

Active Publication Date: 2018-01-05
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the above methods have some limitations, including the need to use large equipment, expensive reagents, etc., so it is still necessary to develop new detection methods with high sensitivity and low cost

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  • RNA aptamer-based theophylline quantitative detection method
  • RNA aptamer-based theophylline quantitative detection method
  • RNA aptamer-based theophylline quantitative detection method

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Embodiment Construction

[0028] The present invention will be further described in detail below in conjunction with the embodiments, so that those skilled in the art can implement it with reference to the description.

[0029] This case lists the quantitative detection method of theophylline based on RNA aptamer of an embodiment, and its principle is as follows figure 1 As shown, the method includes:

[0030] Two RNA probes are used to specifically capture theophylline and form a sandwich structure. One RNA probe 1 is also used to bind to the electrode, and the other RNA probe 2 is used to bind to electrical signal molecules; The content of theophylline is obtained from the chemical response value; wherein, the two RNA probes have fragments that can be complementary to each other, so that the sandwich fragments can be connected end-to-end and finally form a long linear chain.

[0031] RNA probe 1 binds to the electrode through a molecular assembly interface. The electrodes are preferably gold electr...

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Abstract

The invention discloses a RNA aptamer-based theophylline quantitative detection method, which comprises the following steps: two RNA probes are adopted to specifically capture theophylline and a sandwich structure is formed, wherein one RNA probe 1 is also used for combining an electrode, and the other RNA probe 2 is combined with electric signal module; and content of theophylline is obtained bymeasuring electrochemical response value of the modified electrode, wherein the two RNA probes have fragments capable of complementary pairing such that sandwich fragments can be connected in series end to end to finally form a linear long-chain. By combining specific recognition function of the aptamer, molecular interface assembling function of DNA tetrahedron and electrochemical stripping voltammetry performance of silver nanoparticles, high-sensitivity, high-specificity and low-interference electrochemical detection of theophylline is realized.

Description

technical field [0001] The invention relates to a detection method of theophylline, in particular to an electrochemical quantitative detection method of theophylline based on RNA aptamer. Background technique [0002] Theophylline is 1,3-dimethyl-3,7-dihydro-1H-purine-2,6-dione, the appearance is white crystal or crystalline powder, odorless, bitter taste, its molecular formula is C 7 h 8 N 4 o 2 , the molecular weight is 180.16, the boiling point is 390.1°C (760mmHg), and the vapor pressure is 2.72E-06mmHg (25°C). It is widely used clinically to treat diseases such as asthma, chronic obstructive pulmonary disease and apnea of ​​premature infants. In addition, theophylline also promotes the release of catecholamines and regulates the influx of calcium ions. [0003] However, the pharmacokinetics of theophylline is easily affected by many factors, and there are large individual differences. When the drug dosage is large and the blood concentration is high, it often sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N27/327G01N27/48
Inventor 缪鹏陈锡峰杨大威郭振振孟凡渝
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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