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Glioma diagnostic marker circ2:23823258|23823569 and its application

A technology of glioma and exosomes, which is applied in biochemical equipment and methods, microbiological measurement/testing, DNA/RNA fragments, etc., can solve the problems of poor postoperative survival rate, poor treatment effect, high recurrence rate, etc. To achieve the effect of quickly and accurately grasping the patient's condition, high diagnostic and prognostic analysis value, and convenient and accurate diagnosis

Active Publication Date: 2020-06-05
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are various treatment techniques for glioma, but due to the characteristics of fast tumor infiltration and invasion, and insensitivity to apoptosis mediated by radiotherapy and chemotherapy, the overall treatment effect is not good, the recurrence rate is high, and the prognosis is poor. Therefore, new treatments are sought. Targets and prognostic indicators are critical
At present, the diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the postoperative survival rate is not optimistic

Method used

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  • Glioma diagnostic marker circ2:23823258|23823569 and its application
  • Glioma diagnostic marker circ2:23823258|23823569 and its application
  • Glioma diagnostic marker circ2:23823258|23823569 and its application

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Embodiment Construction

[0019] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.

[0020] 1. Research object

[0021] 1. The two glioma cell lines are U251 and U87 cell lines, and the six primary glioma cell lines are 1104, 1216, 1125, 1124B, 1124C, and 0128C, all provided by the Institute of Cancer, School of Basic Medicine, Central South University.

[0022] 2. The serum samples of 40 patients with glioma were provided by Xiangya Hospital, and the serum samples of 15 normal patients were healthy individuals who underwent community disease screening at the same time. The samples used for the research were collected at the same period, and the conditions of sampling, packaging and storage were the same.

[0023] 2. Research Methods

[0024] 1. Extraction of RNA from cells, exosomes secreted by cells, and glioma / normal serum exosomes

[0025] A. Extraction of cellular RNA

[0026] After the above eight kinds of cells grow t...

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Abstract

The invention discloses a glioma diagnostic marker Circ2: 23823258|23823569 and an application. According to the invention, it is first found that an exosome secreted by glioma cells has an obvious enrichment effect on Circ2: 23823258|23823569, and the expression level of Circ2: 23823258|23823569 in the serum exosome of a patient who suffers from glioma is obviously improved (p<0.0001) than that of a control group. ROC curve analysis shows that the marker has relatively high diagnostic value (AUC=0.811, p=0.002, the susceptibility is 66.7%, and the specificity is 91.7%) on glioma. Therefore, through detection of the expression level of Circ2: 23823258|23823569 in the serum exosome of a patient who suffers from glioma, noninvasive diagnosis can be early fast made to the patient who suffersfrom glioma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum circRNA marker Circ2:23823258|23823569 for glioma diagnosis, and the application of a reagent for detecting the marker to prepare a glioma diagnostic preparation, and a kit. Background technique [0002] Glioma is the most common malignant neuroepithelial cell tumor in the brain, accounting for 40.49% of intracranial tumors, and is one of the most common brain tumors in adults. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. At present, there are various treatment techniques for glioma, but due to the characteristics of fast tumor infiltration and invasion, and insensitivity to apoptosis mediated by radiotherapy and chemotherapy, the overall treatment effect is not good, the recurrence rate is high, and the prognosis is poor. Therefore, new treatments are sought. Targets and prognostic indicators are critical. At presen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
Inventor 武明花汤颖李沛瑶刘庆袁健赵春花刘洋
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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