Glioma prognostic marker circ7:73686636|73687095 and application

A diagnostic marker and glioma technology, applied in the direction of biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problem of patients with short survival, high invasiveness, and glioma There is no obvious improvement in the survival rate, etc., to achieve the effect of convenient and accurate diagnosis and improve the effect of clinical treatment

Active Publication Date: 2018-04-20
XIANGYA HOSPITAL CENT SOUTH UNIV
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Problems solved by technology

Due to its high invasiveness and high recurrence rate, the cure rate of glioma is low and the survival period of patients is short
The continuous progress of neuroimaging technology and microsurgery technology has greatly promoted the development of neurosurgery, and the continuous deepening of molecular biology research has provided broad prospects for gene therapy of tumors. rate did not increase significantly

Method used

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  • Glioma prognostic marker circ7:73686636|73687095 and application
  • Glioma prognostic marker circ7:73686636|73687095 and application

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Experimental program
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Embodiment Construction

[0019] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.

[0020] 1. Research object

[0021] The serum samples of 40 patients with glioma were provided by Xiangya Hospital, and the serum samples of 19 normal patients were healthy individuals who underwent community disease screening at the same time. The samples used for the research were collected at the same period, and the conditions of sampling, packaging and storage were the same.

[0022] 2. Research methods

[0023] 1. Extraction of RNA from Glioma / Normal Serum Exosomes

[0024] Take 200 μl of serum and centrifuge at 2000 g for 30 minutes at room temperature, use a micropipette to extract the supernatant into a new 600 μl centrifuge tube, add 40 μl of exosome extraction reagent (Total Exosome Isolation Reagent (from serum), product number 4478360, Invitrogen Company) gently Shake gently up and down, and incubate at 4°C for 45 minutes. After ...

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Abstract

The invention belongs to the biotechnology field, and discloses a glioma prognostic marker circ7:73686636|73687095 and application. In the invention, it is firstly found that the expression level of circ7:73686636|73687095 in the serum exosome of a glioma patient is obviously increased compared with a control group (p is equal to 0.0012), and ROC analysis shows that the glioma prognostic marker circ7:73686636|73687095 has a relatively high diagnostic value for a glioma (AUC is equal to 0.872, P is equal to 0.001, the sensitivity is 76.5% and the specificity is 90.9%). Therefore, an early-stagerapid non-invasive diagnosis of the glioma patient can be made by detecting the expression level of circ7:73686636|73687095 in the serum exosome of the glioma patient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum circRNA marker circ7:73686636|73687095 for glioma diagnosis, and the application of a reagent for detecting the marker for preparing glioma diagnostic preparations, and a kit. Background technique [0002] Glioma is the most common malignant tumor of the central nervous system and one of the major diseases of the central nervous system, ranking first in the incidence of primary intracranial tumors. Due to its high invasiveness and high recurrence rate, the cure rate of glioma is low and the survival period of patients is short. The continuous progress of neuroimaging technology and microsurgery technology has greatly promoted the development of neurosurgery, and the continuous deepening of molecular biology research has provided broad prospects for gene therapy of tumors. rate did not increase significantly. Therefore, finding diagnostic markers for glioma to screen high-risk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 武明花王蓉付海娟李沛瑶冯紫阳刘洋赵春花
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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