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Wheat mutation breeding method

A technology for mutation breeding and wheat seeds, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of reducing the incidence of rust disease, and wheat seeds are susceptible to rust disease, so as to achieve a low incidence of rust disease, improve wheat The effect of disease resistance, rooting ability enhancement

Inactive Publication Date: 2018-01-16
当涂县海纳农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] To solve the use of 60 The wheat seeds induced by Co gamma rays all appear to be susceptible to rust, and the present invention selects 60 After the wheat was induced by Coγ-rays, the seedlings were uniformly raised in the seedling room, and the wheat was treated with high and low temperature during the seedling raising period to promote the accumulation of a large amount of total phenolic acids in the wheat root system and improve the ability of the wheat root system to prevent rust infection. Reducing the Incidence of Rust During Wheat Planting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, a kind of wheat mutation breeding method, specifically:

[0017] Wheat Seed 60 After Coγ-ray induction treatment, treat with gibberellin with a concentration of 300mg / L;

[0018] The gibberellin treatment time is 24 hours;

[0019] The seeds treated with gibberellin are subjected to high and low temperature alternate culture;

[0020] During the alternating high and low temperature cultivation process, the low temperature cultivation temperature is 14°C and the time is 11 hours;

[0021] The high-temperature culture temperature is 19°C, and the culture time is 11 hours;

[0022] The high and low temperature alternating culture time is 4 days;

[0023] The wheat obtained by high and low temperature treatment was transplanted to the field for fixed value.

[0024] The object of treatment is Wanmai 52, mutagenic factors and exposure dose: 60 Co gamma ray 30Gy, the wheat seed after mutagenesis treatment is randomly divided into experimental group, contro...

Embodiment 2

[0028] Embodiment 2, a kind of wheat mutation breeding method, specifically:

[0029] Wheat Seed 60 After Coγ-ray induction treatment, treat with gibberellin with a concentration of 400mg / L;

[0030] The gibberellin treatment time is 24 hours;

[0031] The seeds treated with gibberellin are subjected to high and low temperature alternate culture;

[0032] During the high and low temperature alternating culture process, the low temperature culture temperature is 16°C, and the time is 13 hours;

[0033] The high-temperature culture temperature is 21°C, and the culture time is 13 hours;

[0034] The high and low temperature alternating culture time is 5 days;

[0035] The wheat obtained by high and low temperature treatment was transplanted to the field for fixed value.

[0036] The object of treatment is Wanmai 50, mutagenic factors and exposure dose: 60 Co gamma ray 30Gy, the wheat seed after mutagenesis treatment is randomly divided into experimental group, control group...

Embodiment 3

[0040] Embodiment 3, a kind of wheat mutation breeding method, specifically:

[0041] Wheat Seed 60 After Co gamma ray-induced treatment, it was treated with gibberellin with a concentration of 330 mg / L;

[0042] The gibberellin treatment time is 24 hours;

[0043] The seeds treated with gibberellin are subjected to high and low temperature alternate culture;

[0044] During the high and low temperature alternating culture process, the low temperature culture temperature is 15°C, and the time is 12 hours;

[0045] The high-temperature cultivation temperature is 20°C, and the cultivation time is 12 hours;

[0046] The high and low temperature alternating culture time is 5 days;

[0047] The wheat obtained by high and low temperature treatment was transplanted to the field for fixed value.

[0048] The treatment object is Huaimai 22, mutagenic factors and exposure dose: 60 Co gamma ray 30Gy, the wheat seed after mutagenesis treatment is randomly divided into experimental g...

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Abstract

The invention relates to the technical field of biological breeding, and in particular, relates to a wheat mutation breeding method. The wheat mutation breeding method specifically comprises the steps: carrying out 60Co gamma ray induced treatment on wheat seeds, then treating with gibberellin with the concentration of 300-400 mg / L, and carrying out high and low temperature alternative culture ofthe gibberellin treated seeds, wherein in the process of high and low temperature alternative culture, the low-temperature culture temperature is 14-16 DEG C, the culture time is 11-13 hours, the high-temperature culture temperature is 19-21 DEG C, and the culture time is 11-13 hours; and transplanting the wheat obtained by high and low temperature treatment to a field, and fixedly planting. The problem that all the wheat seeds obtained by 60Co gamma ray induction are susceptible to infection of rust disease is effectively solved, the wheat obtained by breeding is transplanted to the field, and the rust disease occurrence rate is less than 0.4% in the premise without rust prevention and control.

Description

technical field [0001] The invention relates to the technical field of biological breeding, in particular to a wheat mutation breeding method. Background technique [0002] Wheat mutation breeding is the artificial use of physical mutagenic factors (such as X-rays, γ-rays, β-rays, fast neutrons, lasers, electron beams, ion beams, ultraviolet rays, etc.) nitrogen compounds, base analogs, etc.) to induce genetic variation in wheat, obtain useful mutants in a relatively short period of time, and according to the requirements of breeding objectives, select new germplasm and use them as parents in breeding (that is, mutants are indirectly using) breeding approach, the current wheat mutagenic factors are mainly 60 Coγ-rays, different exposure doses have different specific induction effects, but the general 60 Wheat seeds induced by Coγ-rays are susceptible to rust. At present, there is no good control method except chemical means for control during planting. Contents of the in...

Claims

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Application Information

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IPC IPC(8): A01H1/06
Inventor 胡晨
Owner 当涂县海纳农业科技有限公司
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