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A Breeding Method for a Restorer Line of Dominant Genic Sterility in Brassica napus

A cabbage rape, nuclear sterility technology, applied in the directions of botanical equipment and methods, plant genetic improvement, application, etc., can solve problems such as low regeneration frequency, and achieve the advantages of shortening the breeding period, improving the embryo production rate, and accelerating the breeding process. Effect

Active Publication Date: 2019-12-10
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the embryoid body regeneration frequency of Brassica napus microspores is affected by many factors, and the regeneration frequency is still low

Method used

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  • A Breeding Method for a Restorer Line of Dominant Genic Sterility in Brassica napus
  • A Breeding Method for a Restorer Line of Dominant Genic Sterility in Brassica napus
  • A Breeding Method for a Restorer Line of Dominant Genic Sterility in Brassica napus

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Comparison scheme
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Embodiment

[0033] Embodiment A kind of breeding method of dominant sterility restorer line of Brassica napus

[0034] 1.F 1 generational acquisition

[0035] The homozygous two-type sterile line HY15AB with the genotype MsMsrfrf was used as the female parent, and the dominant sterility restorer line HF06 with the genotype MsMsRfRf was used as the male parent, and crossed to obtain F with the genotype MsMsRfrf. 1 generation seeds.

[0036] HY15AB is a new homozygous two-type line of dominant genic male sterility selected and bred by researcher Zhou Xirong of Shanghai Academy of Agricultural Sciences in 2010. Its acquisition process and characteristics are as follows:

[0037] The double-low rapeseed variety "Huyou 15" was used as the recurrent parent, and it was crossed with the sterile line of the dominant genic sterile double-low homozygous type "48AB" and the double-low restorer line "PG-Hua" respectively, and two cycles were carried out. Then, the Ms sterility gene and the Rf resto...

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Abstract

A breeding method of dominant genic male sterility restorer of brassica napus comprises the steps as follows: an excellent homozygotic sterile plant serving as a female parent and a restorer serving as a male parent are hybridized, and an F1 generation is obtained; then, free microspores of the F1 generation plant are subjected to induction culture with a brassica napus microspore culture technology, and a homozygotic diploid DH strain is obtained; successfully induced DH strain seedlings are transplanted to a field, fertile DH strains are bagged for selfing in the flowering period, and highlyhomozygous dominant genic male sterility restorer seeds with the genotype of MsMsRfRf are obtained. The method is simple to operate, high in feasibility, short in breeding cycle and high in efficiency and can be well applied to dominant genic male sterility crossbreeding of brassica napus.

Description

technical field [0001] The invention relates to the field of Brassica napus breeding, in particular to a method for selecting a dominant sterility restorer line of Brassica napus. Background technique [0002] Brassica napus dominant genic male sterile three lines include homozygous two-type line, temporary protection line and restorer line, wherein, the genotype of the restorer line is RfRf, which is fertile and has no performance traits that can be used for selection. [0003] In conventional cross-breeding, it usually takes 5 to 8 generations of trait selection to obtain stable genetic offspring or lines. The traditional breeding of Brassica napus restorer lines starts from the first generation of backcrossing, self-crossing the selected plants, and at the same time using the homozygous male sterile plants of dominant genic sterility as the female parent, and testcrossing the selected individual plants to finally obtain The genotype is the restorer line of RfRf. However,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 张俊英周熙荣蒋美艳杨立勇李延莉江建霞王伟荣孙超才顾龙弟
Owner SHANGHAI ACAD OF AGRI SCI