Induction and regeneration rapid breeding method of paeonia suffruticosa

Abstract

The invention discloses an induction and regeneration rapid breeding method of paeonia suffruticosa. The method comprises the following steps: in the morning of a sunny day, obtaining a stem segment of paeonia suffruticosa, removing dirt, stripping roots, soaking, cleaning, sterilizing, then cutting into small sections of callus, inoculating into an induction culture medium to perform the induction cultivation under an aseptic condition, growing a cluster bud, dividing into single buds, transferring the single buds onto a rooting culture medium to increase the roots, then soaking in a calciumchloride solution, transplanting in a combination manner, performing the preliminary cultivation under a complete dark condition after the transplanting, then performing the normal lighting, sproutingleaves, and applying the fertilizer. By treating the paeonia suffruticosa explants, inducing breeding the callus, dividing, rooting, transplanting rooted seedlings, and maintaining the rooted seedlings, the paeonia suffruticosa callus can be rapidly and effectively induced into cluster buds in a short period of time, the induced buds are rooted, the survival rate after the transplanting is high,the production period is short, and the method is simple and easy.

Description

technical field [0001] The invention belongs to the breeding field of peony, and in particular relates to a rapid breeding method for induction and regeneration of peony. Background technique [0002] my country is the hometown of peonies, and the germplasm resources are very rich. Most of them are concentrated in the high mountains in the southwest of my country and the Loess Plateau and hilly areas in the north and middle of China. They are typical temperate tree species. Peony has a long history of cultivation. During the Southern and Northern Dynasties, peony was transformed from wild state to artificial cultivation. After a long period of natural selection and artificial breeding, a group of peony varieties with wide adaptability and rich flower colors and patterns were cultivated. According to the different wild original species and peony cultivation areas, it can be divided into the Central Plains variety group, the Jiangnan variety group, the Northwest variety group ...

AI Technical Summary

Problems solved by technology

However, the traditional breeding method has a low multiplication coefficient, slow seedling growth rate, seasonal restrictions, and long breeding cycle for new varieties, resulting in low reproduction rate of famous peony varieties, which seriously restricts the breeding process, commercialization and industrialization of fine peony varieties. Production