Method for artificially feeding and breeding corn aphides by the aid of living corn plants

Abstract

The invention discloses a method for artificially feeding and breeding corn aphides by the aid of living corn plants. The method includes: selecting formula soil obtained by mixing soil with nutrientsoil as a culture matrix for the living corn plants; sowing seeds of aphid-susceptible corn varieties in the culture matrix, and then cultivating the corn plants in a manual climatic box; inoculatingcorn aphid sources when the corn plants grow to have two leaves and one core, and performing feeding and breeding of the corn aphides under the premise of cultivating the corn plants continuously in the manual climatic box. With the method, the breeding process of the corn aphides is close to the natural state, a large number of corn aphid samples, with physiological and biochemical characteristics with identical biological characteristics, hereditary characteristics and the like, identical to naturally captured corn aphides are obtained, supply of the insect source samples is more stable andhas more universal significance, and great significance is achieved for detailed and accurate biological research of the aphides, precise location cloning research of aphid-resistant genes, molecularmark assisted breeding and selection and breeding of fine anti-aphid corn varieties.

Description

technical field [0001] The invention relates to the technical field of artificial cultivation of corn aphids, in particular to a method for artificially raising and propagating corn aphids by using living corn plants. Background technique [0002] Aphids are extremely common crop pests with a wide host range that can cause damage to almost all major cultivated crops. Aphids belong to the family Aphididae of the order Homoptera and are commonly known as greasy insects or dense insects. There are nearly 5,000 species of aphids in the world, among which the major damage to corn is corn aphid (Rhopalosiphum maidis), followed by sorghum aphid (Melanaphis sacchari). Corn aphids are widely distributed in Northeast, Northwest, North and Central China, and their host plants are diverse, including not only corn, sorghum, rice, wheat and other gramineous crops, but also barnyardgrass, crabgrass, horsetail and other weeds. The adults and nymphs of corn aphids directly damage crops by ...

AI Technical Summary

Problems solved by technology

Corn aphids can have more than 10 generations in northern my country every year, and overwinter as wingless viviparous female aphids on wheat seedlings and grass weeds; winged aphids migrate to spring corn and sorghum fields at the end of April and early May to cause damage; During the reproductive growth period of sowing corn, it spreads to the summer sowing corn field. At this time, the number of insects doubles and enters the stage of serious damage; in the first and middle of October, the corn gradually ages, and the corn aphid produces winged aphids for the last time, and moves into the wheat field to cause damage Therefore, the collection of corn aphids in the field is affected by many factors such as aphid migration, season, weather, etc., making the identification efficiency of aphid resistance low and the results unreliable

For the artificial breeding of aphids, solid medium is often used to raise them in fixed containers at present, such as Chinese patent application CN201710099350.1. Although this breeding method can successfully obtain the reproduction of aphids, the obtained aphid samples are directional induced by the artificial feeding method. There are differences in biological, genetic, and genetic characteristics between aphid samples captured in the natural environment, resulting in large differences between research results and natural control effects, which is not conducive to practical application

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